In this class, students engage in independent research projects to probe various aspects of the physiology of the bacterium Pseudomonas aeruginosa PA14, an opportunistic pathogen isolated from the lungs of cystic fibrosis patients. Students use molecular genetics to examine survival in stationary phase, antibiotic resistance, phase variation, toxin production, and secondary metabolite production.
Projects aim to discover the molecular basis for these processes using both classical and cutting-edge techniques. These include plasmid manipulation, genetic complementation, mutagenesis, PCR, DNA sequencing, enzyme assays, and gene expression studies. Instruction and practice in written and oral communication are also emphasized.
WARNING NOTICE
The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such safety procedures and measures. MIT shall have no responsibility, liability, or risk for the content or implementation of any of the material presented.
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A single lecture given at Kwame Nkrumah University of Science and Technology about bacterial vaginosis, HACEK infections, and legionella.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Blue-green algae, or cyanobacteria, are photosynthetic bacteria that live in water and are important oxygen producers. Human-driven changes are a major factor causing seasonal cyanobacterial blooms, which can cause mass death of aquatic animals. Bacteria-infecting viruses, called phages, could potentially be used to control these outbreaks with minimal environmental disruption. But to date few freshwater ‘cyanophages’ have been isolated or had their genomes sequenced. Recently, researchers isolated a strain of cyanobacteria from Lake Chaohu, a massive lake in China with seasonal cyanobacterial blooms. Using the new cyanobacteria strain, they isolated five new freshwater cyanophages with varying tail structures from the same lake. While viruses can have RNA genomes or even single-stranded DNA genomes, all five isolated phages had double-stranded DNA genomes. Further analysis suggested that they all use different DNA packaging mechanisms and are evolutionarily distinct..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

This is a short activity for growing the slime mold Physarum polycephalum. It includes materials needed, a three-step procedure, and helpful tips. The site is part of the Turbulent Landscapes exhibit at the Exploratorium. Links to other activities and features of the exhibit are also included.

This resource is a collection of articles, book chapters, and videos about the Human Microbiome.The Microbiome is loosely defined as microorganisms, such as bacteria, that are found throughout the human body. It plays an important role in our understanding of our interactions with microorganisms and can help better understand which microorganisms are associated with clinical conditions and can help to improve the overall state of human health. The Human Microbiome provides some background information on microorganisms in general. There is a lot of Microbiome information provided. Some in the form of informative video content, some in the form of an online course at MIT and links to papers and online books and other important websites that inform a lot about the microbiome.  Finally, since this is intended to be a resource for Lander College for Women, a Womens Jewish College, there is also information about the impact of the human microbiome on women's health, as well as information regarding a parallel concept in Jewish Philosophy, that a human being is a microcosm of a world.-Neil Normand, Touro University, 2021

This set of lecture materials, laboratory notes, and activities are ancillary materials created for use with OpenStax Microbiology. The materials were created under a Round Nine Textbook Transformation Grant.

Topics covered include:

The Cell
Prokaryotes
Eukaryotes
Acellular Pathogens
Microbial Metabolism
Microbial Growth
Biochemistry of the Bacterial Genome
Microbial Genetics
Microbial Growth
Antimicrobial Drugs
Pathogenicity
Epidemiology
Immune System

Students will investigate arctic geology and hydrology as well as tundra ecology as they consider options for sewage treatment. Public safety, environmental impact, and issues of construction and engineering will be explored.

(Note: this resource was added to OER Commons as part of a batch upload of over 2,200 records. If you notice an issue with the quality of the metadata, please let us know by using the 'report' button and we will flag it for consideration.)

The Los Angeles and the Future of Mono Lake WebQuest leads students in a guided exploration of Mono Lake's extreme environment and asks them to consider the preservation of this environment in relation to the needs of humans.

(Note: this resource was added to OER Commons as part of a batch upload of over 2,200 records. If you notice an issue with the quality of the metadata, please let us know by using the 'report' button and we will flag it for consideration.)

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Ruminants are the only animals not dependent on dietary amino acids as a source of nitrogen. They have ureolytic bacteria in their rumen that hydrolyze urea into ammonia and use it as a nitrogen source. However, very few ureolytic bacteria have been isolated and studied in pure culture to date. To close this gap, researchers established and used a new integrated approach on bacteria from cattle rumens. They started with urease gene (ureC) guided enrichment and then embedded single cells in agarose microspheres for in situ cultivation. This allowed them to isolate and characterize diverse ureolytic bacteria with demonstrated urease activity. The researchers sequenced a subset of the isolated bacteria and found 28 strains from 12 species with urease genes. These bacterial species had not previously been found in the rumen, but this team detected them in metagenomes from 6 ruminant species. The new strains contained unique genes compared to known related strains, indicating new metabolic functions..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

The pages represent an online biology textbook created by Professor John W. Kimball who taught at Harvard, Tufts, and Phillips Andover Academy before he retired. He now maintains this free biology book, constantly updated and set up for easy browsing and searching.

This seminar is designed to be an experimental and hands-on approach to applied chemistry (as seen in cooking). Cooking may be the oldest and most widespread application of chemistry and recipes may be the oldest practical result of chemical research. We shall do some cooking experiments to illustrate some chemical principles, including extraction, denaturation, and phase changes.

This laboratory manual was developed for a microbiology laboratory course designed to give students in the health care professions basic knowledge and skills of the techniques used to study microbes. The course is taken by first-time college students in the 6-year medical program and by prepharmacy undergraduates. Because this is the only microbiology laboratory course these students take, the laboratory experiments are essential to illustrate microbiological principles and methods presented in lecture companion course. The laboratory exercises demonstrate basic concepts of microbiology with emphasis on infectious diseases and host defenses.

Throughout the course, students gain competency in the following areas:

Safe handling of microbes
Knowledge of the techniques and media used to subculture microbes
Use of the light microscope
Staining techniques
Quantitative methods
Identification of microbes using biochemical tests and/or immunological techniques
Interpretation of experimental results

In the past, I supplemented a commercially published lab manual with detailed weekly instructions posted to the course website. My instructions summarized the theory presented, pointing out the important concepts. Based on past experience, I made changes to the lab procedure accommodating organisms that work well in the UMKC teaching lab. In addition, the instructions gave students clarification on the post lab questions, encouraging critical thinking and evaluation of their actual experimental results. Students were required to use both the manual and my handouts to fully understand the exercise. As much as I tried to make each week’s activities clear, there was often confusion about the procedure, observations and/or expectations on the post-lab questions. This work aims to put it all together in one place for the student. For this project, I have built upon much of my original supplementary material using several open educational resources, most notably, OpenStax Microbiology. I appreciate the funding and support from the UM-system and the UMKC Libraries. I am grateful to my students who make teaching fun and interesting and will be unwitting editors and evaluators of this work. Sincerely,

Loretta Sanderson Klamm

The exercises in this laboratory manual are designed to engage students in hand-on activities that reinforce their understanding of the microbial world. Topics covered include: staining and microscopy, metabolic testing, physical and chemical control of microorganisms, and immunology. The target audience is primarily students preparing for a career in the health sciences, however many of the topics would be appropriate for a general microbiology course as well.

This comprehensive module explores common laboratory experiments for microbiology, including microbiological stains (e.g. Gram stains), assessing the accuracy of diagnostic tests, measuring antibody response to infection, and Detection of microbial antigens, and nucleic acid amplification (e.g. Polymerase Chain Reaction).

Here you find 9 different INTERACTIVE VIDEO-QUIZZES that will help students with training laboratory safety.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Advances in genomic laboratory and bioinformatics techniques have allowed us to infer microbial ecology information from genomes. This ability has led to great advances in microbiome science; however, there is not yet a standard comprehensive workflow for functional annotation. Some software tools annotate metabolic functions, but the new tool 'METABOLIC' improves upon this and expands into biogeochemical pathways like the carbon cycle. METABOLIC takes sequence inputs from isolates, metagenome-assembled genomes, or single-cell genomes. The data can be processed through two workflow scales: genome and/or community. The genome-scale workflow annotates the genomes and validates motifs of conserved protein residues. It also analyzes metabolic pathways and calculates the microbial contributions to individual biogeochemical processes and cycles. The community-scale workflow adds to this by first determining the genome abundance in the microbiome..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

This course is the first in a three-course sequence that introduces biology in preparation for advanced study in areas of biological science such as medicine, dentistry, cell biology, microbiology, or veterinary medicine. Biol& 211 introduces students to cellular structure and function. Major topics studied include: energy capture and utilization, cellular reproduction, inheritance, genetic mutation, protein synthesis, gene expression, and biotechnology.

In MedMyst: Disease Defenders, players can choose to train with an epidemiologist, microbiologist, or veterinarian to learn how these experts work as a team to solve infectious disease outbreaks while using the scientific method. Each expert path has its own learning objectives and stresses different parts of the scientific method.