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How Enzymes Work: Investigating Their Specificity and Susceptibility to Environmental Factors Using Jell-O
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This activity is a lab investigation in which students design and conduct ...

This activity is a lab investigation in which students design and conduct experiments using pineapple juice containing the enzyme bromelain and its affect on the substrate gelatin found in Jell-O. The focus of student driven investigations are on enzyme specificity, activity and the impact of environmental factors on enzyme functioning. Based on the original activities from School Improvement in Maryland; "Pineapple/Jell-O Lab," Access Excellence Activities Exchange; "Enzyme Labs Using Jell-O" by Anne McDonald and Michael O'Hare, and AP & Regents Biology; "Lab 8: Pineapple Enzymes and Jell-O Molds" by Kim B. Foglia.

Subject:
Education
Life Science
Biology
Science and Technology
Material Type:
Activities and Labs
Assessments
Instructional Material
Lesson Plans
Provider:
Science Education Resource Center (SERC) at Carleton College
Provider Set:
Pedagogy in Action
Author:
Heather Netland, Jefferson High School, Alexandria, MN, based on the original activities from School Improvement in Maryland; "Pineapple/Jell-O Lab," Access Excellence Activities Exchange; "Enzyme Labs Using Jell-O" by Anne McDonald and Michael O'Hare, and AP & Regents Biology; "Lab 8: Pineapple Enzymes and Jell-O Molds" by Kim B. Foglia.
Netland, Heather
Human DNA Fingerprinting by Polymerase Chain Reaction
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This experiment uses polymerase chain reaction to demonstrate the polymorphic nature of ...

This experiment uses polymerase chain reaction to demonstrate the polymorphic nature of human DNA. Students obtain samples of their own DNA using a simple mouthwash procedure. PCR is used to amplify a noncoding region of chromosome 1 that contains a repeated DNA sequence. The number of times the sequence repeats can vary from person to person, resulting in a polymorphism. Following amplification, student samples are electrophoresed, stained, and photographed. Each student will see one or two bands in their gel lane, indicating whether they are homozygous or heterozygous for that region of chromosome 1. This experiment is adapted from Advanced DNA Science: An Introduction to Methods of Genome Analysis by Mark V. Bloom, Greg A. Freyer, and David A. Micklos (copyright 1993 Cold Spring Harbor Laboratory and Carolina Biological Supply Company); polymerase chain reaction is covered by patents owned by Hoffman La Roche.

Subject:
Biology
Material Type:
Activities and Labs
Lesson Plans
Provider:
Association for Biology Laboratory Education (ABLE)
Author:
Mark Bloom
Immunocytochemical Analysis of Sperm Cytoskeleton
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Monoclonal antitubulin antibodies produced in mice will be used to label bull ...

Monoclonal antitubulin antibodies produced in mice will be used to label bull sperm microtubules and the location of these antibodies will be determined by use of a peroxidase conjugated secondary antibody and the DAB staining reaction. Protocols are presented for use in a second-year student laboratory class given in a multi-section (repeated) format. These protocols include those for preparation of poly-L-lysine coated slides and of bull sperm, as well as those for immunocytochemistry, using the DAB reaction.

Subject:
Biology
Material Type:
Activities and Labs
Lesson Plans
Provider:
Association for Biology Laboratory Education (ABLE)
Author:
Beth Retallack
Incorporating Original Genomics Research into Genetics and Molecular Biology Courses
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Genomics and bioinformatics have revolutionized biology over the past few years, but ...

Genomics and bioinformatics have revolutionized biology over the past few years, but their impact on education has lagged. A new revolution is possible in the undergraduate curriculum. We have found novel ways to incorporate genomics research into the laboratory components of several courses. In this chapter, we show how we accomplish the typical skill development goals in a genetics or molecular biology course while accomplishing novel research, and provide the protocols for three such research projects that can be modified to suit the course goals or the instructor's research interests.

Subject:
Biology
Material Type:
Activities and Labs
Lesson Plans
Provider:
Association for Biology Laboratory Education (ABLE)
Author:
Brad Goodner
Kathy Wheeler
Introducing Students to Conservation Genetics Using Sturgeon Caviar and Other Fish Eggs
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In this laboratory exercise students will learn how to: (a) Isolate DNA ...

In this laboratory exercise students will learn how to: (a) Isolate DNA from individual sturgeon and other fish eggs (available at any local deli that sells caviar) using the DNAzol method, (b) Set up control and species-specific PCR reactions using primers that have been developed for DNA from sturgeon species and (c) Use electrophoresis and methylene blue and/or ethidium bromide staining to visualize the PCR products. This laboratory exercise would allow students to contribute to a growing DNA database on endangered species.

Subject:
Biology
Material Type:
Activities and Labs
Lesson Plans
Provider:
Association for Biology Laboratory Education (ABLE)
Author:
Claire Leonard
Kathleen A. Nolan
Nancy J. Rosenbaum
Phaedra Doukakis
Rob DeSalle
Tony Catalano
Vadim Birstein
Introduction to Biology, Fall 2004
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The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover ...

The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. Biological function at the molecular level is particularly emphasized and covers the structure and regulation of genes, as well as, the structure and synthesis of proteins, how these molecules are integrated into cells, and how these cells are integrated into multicellular systems and organisms. In addition, each version of the subject has its own distinctive material.

Subject:
Education
Life Science
Biology
Genetics
Science and Technology
Material Type:
Activities and Labs
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Provider:
Internet Scout Project
M.I.T.
Provider Set:
Internet Scout Project
M.I.T. OpenCourseWare
An Introduction to DNA: Spectrophotometry, Degradation, and the 'Frankengel' Experiment
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In this laboratory students perform three exercises as an introduction to the ...

In this laboratory students perform three exercises as an introduction to the basics of handling and analyzing DNA. In the first, they expose circular plasmid, linear phage, and high molecular weight genomic DNA samples to a variety of physical, thermal, chemical, and enzymatic conditions that might be expected to affect DNA integrity. The DNA's are analyzed by electrophoresis on a group agarose gel. In the second, they pour and reconstruct a "Frankengel" (a gel containing sections with three different agarose concentrations) on which they run a DNA ladder in order to investigate the effect of gel pore size on DNA fragment mobility. In the third, they perform an UV spectrophotometric analysis of DNA from which they learn to make purity and concentration estimates.

Subject:
Biology
Material Type:
Activities and Labs
Lesson Plans
Provider:
Association for Biology Laboratory Education (ABLE)
Author:
Kimberley Christopher
William Clark
Introduction to Molecular and Cellular Biology
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Though biology as we know it today is a relatively new field, ...

Though biology as we know it today is a relatively new field, we have been studying living things since the beginning of recorded history. This introductory course in biology starts at the microscopic level, with molecules and cells, then moves into the specifics of cell structure and behavior. Upon successful completion of this course, students will be able to: Describe in general terms how life began on Earth; Identify early scientists that played important roles in furthering our understanding of cellular life; Describe the characteristics that define life; List the inorganic and organic molecules that are necessary for life; List the structure and function of organelles in animal and plant cells; List the similarities and differences between animal and plant cells; Describe the reactions in photosynthesis; Explain how the different photosynthetic reactions are found in different parts of the chloroplast; Describe the sequence of photosynthetic reactions; Explain the use of products and the synthesis of reactants in photosynthesis; Explain how protein is synthesized in eukaryotic cells; Describe the similarities and differences between photosynthesis and aerobic respiration; List the reactions in aerobic respiration; Explain the use of products and the synthesis of reactants in aerobic respiration; Describe the similarities and differences between anaerobic and aerobic respiration. (Biology 101; See also: Psychology 203)

Subject:
Biology
Genetics
Science and Technology
Material Type:
Assessments
Audio Lectures
Full Course
Lecture Notes
Readings
Syllabi
Video Lectures
Other
Provider:
The Saylor Foundation
Introduction to Molecular and Cellular Biology - Laboratory
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This lab course supplements Introduction to Molecular and Cellular Biology. Although it ...

This lab course supplements Introduction to Molecular and Cellular Biology. Although it does not replicate a true lab experience, it does enable further exploration of some key principles of molecular and cellular biology. In each unit, the student will work through tutorials related to important scientific concepts, and then will be asked to think creatively about how those concepts can be put to practical or experimental use. This lab course also contains activities devoted to learning important techniques in scientific study such as microscope use, DNA extraction, Polymerase Chain Reaction, and examination of DNA microarrays. Upon successful completion of this lab supplement, students will be able to: Identify the important components of scientific experiments and create their own experiments; Identify the molecular differences between proteins, fats, and carbohydrates, and explain the molecular behavior of water; Describe the process of photosynthesis; Describe the process of cellular respiration; Identify the differences between DNA and RNA; Describe the entire transcription/translation process, from gene to protein; Explain how recombinant genomes are formed; Use critical thinking to find ways that any of the above natural processes might be altered or manipulated; Explain how to use a compound light microscope for data collection; Explain how to conduct and use various experimental techniques, including DNA extraction, PCR, and DNA microarrays. (Biology 101 Laboratory)

Subject:
Biology
Genetics
Science and Technology
Material Type:
Activities and Labs
Full Course
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Readings
Provider:
The Saylor Foundation
Investigating Characteristics of Enzymes and the Factors That Affect Their Function
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This classroom lab allows students to develop skills of the scientific method ...

This classroom lab allows students to develop skills of the scientific method while exploring the characteristics of enzymes.

Subject:
Education
Life Science
Chemistry
Science and Technology
Material Type:
Activities and Labs
Assessments
Instructional Material
Lesson Plans
Provider:
Science Education Resource Center (SERC) at Carleton College
Provider Set:
Pedagogy in Action
Author:
Jen Vance
Vance, Jen
Investigating Starch in Foods
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This activity is a classroom experiment where students gather data on which ...

This activity is a classroom experiment where students gather data on which foods contain starch. They use observation and develop their own hypothesis to create a question they would like to discover about starches.

Subject:
Education
Life Science
Nutrition
Science and Technology
Material Type:
Activities and Labs
Instructional Material
Provider:
Science Education Resource Center (SERC) at Carleton College
Provider Set:
Pedagogy in Action
Author:
Darlene Schleis
Kimball's Biology Pages
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The pages represent an online biology textbook created by Prof. Kimball who ...

The pages represent an online biology textbook created by Prof. Kimball who taught at Harvard, Tufts, and Phillips Andover Academy before he retired. He now maintains this free biology book, constantly updated and set up for easy browsing and searching.

Subject:
Biology
Science and Technology
Material Type:
Textbooks
Provider:
Microbial Life (MLER) (SERC)
Science Education Resource Center (SERC) at Carleton College
Author:
John W. Kimball