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Incorporating Original Genomics Research into Genetics and Molecular Biology Courses
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Genomics and bioinformatics have revolutionized biology over the past few years, but ...

Genomics and bioinformatics have revolutionized biology over the past few years, but their impact on education has lagged. A new revolution is possible in the undergraduate curriculum. We have found novel ways to incorporate genomics research into the laboratory components of several courses. In this chapter, we show how we accomplish the typical skill development goals in a genetics or molecular biology course while accomplishing novel research, and provide the protocols for three such research projects that can be modified to suit the course goals or the instructor's research interests.

Subject:
Biology
Material Type:
Activities and Labs
Lesson Plans
Provider:
Association for Biology Laboratory Education (ABLE)
Author:
Brad Goodner
Kathy Wheeler
Introducing Students to Conservation Genetics Using Sturgeon Caviar and Other Fish Eggs
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In this laboratory exercise students will learn how to: (a) Isolate DNA ...

In this laboratory exercise students will learn how to: (a) Isolate DNA from individual sturgeon and other fish eggs (available at any local deli that sells caviar) using the DNAzol method, (b) Set up control and species-specific PCR reactions using primers that have been developed for DNA from sturgeon species and (c) Use electrophoresis and methylene blue and/or ethidium bromide staining to visualize the PCR products. This laboratory exercise would allow students to contribute to a growing DNA database on endangered species.

Subject:
Biology
Material Type:
Activities and Labs
Lesson Plans
Provider:
Association for Biology Laboratory Education (ABLE)
Author:
Claire Leonard
Kathleen A. Nolan
Nancy J. Rosenbaum
Phaedra Doukakis
Rob DeSalle
Tony Catalano
Vadim Birstein
Introduction to Biology, Fall 2004
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The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover ...

The MIT Biology Department core courses, 7.012, 7.013, and 7.014, all cover the same core material, which includes the fundamental principles of biochemistry, genetics, molecular biology, and cell biology. Biological function at the molecular level is particularly emphasized and covers the structure and regulation of genes, as well as, the structure and synthesis of proteins, how these molecules are integrated into cells, and how these cells are integrated into multicellular systems and organisms. In addition, each version of the subject has its own distinctive material.

Subject:
Education
Life Science
Biology
Genetics
Material Type:
Activities and Labs
Assessments
Audio Lectures
Full Course
Homework and Assignments
Syllabi
Video Lectures
Provider:
Internet Scout Project
M.I.T.
Provider Set:
Internet Scout Project
M.I.T. OpenCourseWare
An Introduction to DNA: Spectrophotometry, Degradation, and the 'Frankengel' Experiment
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In this laboratory students perform three exercises as an introduction to the ...

In this laboratory students perform three exercises as an introduction to the basics of handling and analyzing DNA. In the first, they expose circular plasmid, linear phage, and high molecular weight genomic DNA samples to a variety of physical, thermal, chemical, and enzymatic conditions that might be expected to affect DNA integrity. The DNA's are analyzed by electrophoresis on a group agarose gel. In the second, they pour and reconstruct a "Frankengel" (a gel containing sections with three different agarose concentrations) on which they run a DNA ladder in order to investigate the effect of gel pore size on DNA fragment mobility. In the third, they perform an UV spectrophotometric analysis of DNA from which they learn to make purity and concentration estimates.

Subject:
Biology
Material Type:
Activities and Labs
Lesson Plans
Provider:
Association for Biology Laboratory Education (ABLE)
Author:
Kimberley Christopher
William Clark
Introduction to Molecular and Cellular Biology
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Though biology as we know it today is a relatively new field, ...

Though biology as we know it today is a relatively new field, we have been studying living things since the beginning of recorded history. This introductory course in biology starts at the microscopic level, with molecules and cells, then moves into the specifics of cell structure and behavior. Upon successful completion of this course, students will be able to: Describe in general terms how life began on Earth; Identify early scientists that played important roles in furthering our understanding of cellular life; Describe the characteristics that define life; List the inorganic and organic molecules that are necessary for life; List the structure and function of organelles in animal and plant cells; List the similarities and differences between animal and plant cells; Describe the reactions in photosynthesis; Explain how the different photosynthetic reactions are found in different parts of the chloroplast; Describe the sequence of photosynthetic reactions; Explain the use of products and the synthesis of reactants in photosynthesis; Explain how protein is synthesized in eukaryotic cells; Describe the similarities and differences between photosynthesis and aerobic respiration; List the reactions in aerobic respiration; Explain the use of products and the synthesis of reactants in aerobic respiration; Describe the similarities and differences between anaerobic and aerobic respiration. (Biology 101; See also: Psychology 203)

Subject:
Biology
Genetics
Material Type:
Assessments
Audio Lectures
Full Course
Lecture Notes
Readings
Syllabi
Video Lectures
Other
Provider:
The Saylor Foundation
Introduction to Molecular and Cellular Biology - Laboratory
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This lab course supplements Introduction to Molecular and Cellular Biology. Although it ...

This lab course supplements Introduction to Molecular and Cellular Biology. Although it does not replicate a true lab experience, it does enable further exploration of some key principles of molecular and cellular biology. In each unit, the student will work through tutorials related to important scientific concepts, and then will be asked to think creatively about how those concepts can be put to practical or experimental use. This lab course also contains activities devoted to learning important techniques in scientific study such as microscope use, DNA extraction, Polymerase Chain Reaction, and examination of DNA microarrays. Upon successful completion of this lab supplement, students will be able to: Identify the important components of scientific experiments and create their own experiments; Identify the molecular differences between proteins, fats, and carbohydrates, and explain the molecular behavior of water; Describe the process of photosynthesis; Describe the process of cellular respiration; Identify the differences between DNA and RNA; Describe the entire transcription/translation process, from gene to protein; Explain how recombinant genomes are formed; Use critical thinking to find ways that any of the above natural processes might be altered or manipulated; Explain how to use a compound light microscope for data collection; Explain how to conduct and use various experimental techniques, including DNA extraction, PCR, and DNA microarrays. (Biology 101 Laboratory)

Subject:
Biology
Genetics
Material Type:
Activities and Labs
Full Course
Homework and Assignments
Readings
Provider:
The Saylor Foundation
Investigating Characteristics of Enzymes and the Factors That Affect Their Function
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This classroom lab allows students to develop skills of the scientific method ...

This classroom lab allows students to develop skills of the scientific method while exploring the characteristics of enzymes.

Subject:
Education
Life Science
Chemistry
Material Type:
Activities and Labs
Assessments
Instructional Material
Lesson Plans
Provider:
Science Education Resource Center (SERC) at Carleton College
Provider Set:
Pedagogy in Action
Author:
Jen Vance
Vance, Jen
Investigating Starch in Foods
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This activity is a classroom experiment where students gather data on which ...

This activity is a classroom experiment where students gather data on which foods contain starch. They use observation and develop their own hypothesis to create a question they would like to discover about starches.

Subject:
Life Science
Nutrition
Material Type:
Activities and Labs
Instructional Material
Provider:
Science Education Resource Center (SERC) at Carleton College
Provider Set:
Pedagogy in Action
Author:
Darlene Schleis
Kimball's Biology Pages
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No Strings Attached
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The pages represent an online biology textbook created by Professor John W. ...

The pages represent an online biology textbook created by Professor John W. Kimball who taught at Harvard, Tufts, and Phillips Andover Academy before he retired. He now maintains this free biology book, constantly updated and set up for easy browsing and searching.

Subject:
Biology
Material Type:
Textbooks
Provider:
Kimball's Biology Pages
Author:
John W. Kimball
Lighting Up: Analysis Of Yeast RAS Genes Using A Chemiluminescent Southern Blot Procedure
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Genomic and plasmid-encoded RAS2 genes from Baker's and brewing yeast strains will ...

Genomic and plasmid-encoded RAS2 genes from Baker's and brewing yeast strains will be analyzed using a shortened Southern blot procedure employing chemiluminescence. Unique parts of this procedure are the gravity-driven "upside down" transfer and a rotisserie-like hybridization oven, which greatly facilitate shortening the transfer time to 45 minutes and the hybridization step to 60 minutes. Chemiluminescence obviates problems associated with radioisotopes without sacrificing sensitivity: the plasmid-borne RAS gene can be visualized within 15 seconds and the single-copy yeast genomic RAS2 gene, within a minute. Topics for discussion related to this laboratory can include the concepts of RFLPs, evolutionary conservation of DNA sequences and the genetic basis of cancer.

Subject:
Biology
Material Type:
Activities and Labs
Lesson Plans
Provider:
Association for Biology Laboratory Education (ABLE)
Author:
Karen Malatesta
Linda B. Cholewiak
Living With Her Genes: Early Onset Familial Alzheimer's Disease
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When a 30-year-old genetic counselor learns that her 38-year-old sister has developed ...

When a 30-year-old genetic counselor learns that her 38-year-old sister has developed early onset familial Alzheimer's disease (EOFAD), a dominantly inherited disorder that led to their father's death at age 42, she struggles with whether to undergo genetic testing and whether to have children. This interrupted case study examines the impact of genetic testing on people and their families when there is no treatment or cure for a disease. It covers principles of Mendelian inheritance as well as genetic and reproductive technologies ,such as gene tests, pre-implantation genetic diagnosis, and in vitro fertilization. It can be used in introductory biology courses for both majors and non-majors or adapted for more advanced courses in genetics and molecular biology.

Subject:
Life Science
Material Type:
Case Study
Instructional Material
Provider:
National Center for Case Study Teaching in Science
Provider Set:
Case Study Collection
Author:
Alice M. Stanford
Deborah D. Wygal
Lynne H. Gildensoph