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  • Molecular Biology
Expression Vectors Used in Project-Oriented Teaching Laboratories Chapter 2
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This chapter describes a multi-week project taught in an upper level cell ... More

This chapter describes a multi-week project taught in an upper level cell and molecular biology laboratory. Isolation of sufficient quantities of rare proteins from biological tissues can be difficult. These native proteins are often required for raising antibodies, and for studying enzymatic or regulatory function. Expression vectors are used to obtain large quantities of proteins. The cDNA encoding a protein is cloned into an expression vector. The fusion protein is over-expressed in E. coli, and large quantities of the recombinant protein are obtained by affinity chromatography. SDS-polyacrylamide gel electrophoresis is used to determine the size of the proteins isolated. Less

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Subject:
Biology
Material Type:
Activities and Labs
Lesson Plans
Provider:
Association for Biology Laboratory Education (ABLE)
Author:
Chris J. Staiger
David J. Asai
Susan J. Karcher
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Extracting DNA
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This lesson for students in grades 9-12 introduces DNA, genes, chromosomes, the ... More

This lesson for students in grades 9-12 introduces DNA, genes, chromosomes, the chemicals that make up DNA. After the basic information, students will do an experiment in which they will separate out DNA from peas. Knowing that DNA can be separated will give them a base of understanding for future lessons in biology, evolution, biotechnology, and health technology. Less

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Subject:
Biology
Genetics
Material Type:
Lesson Plans
Teaching and Learning Strategies
Provider:
AAAS
Provider Set:
Science Netlinks
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FISH - (Fluoresence In Situ Hybridization)
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Fluorescence in situ hybridization (FISH) is a process which vividly paints chromosomes ... More

Fluorescence in situ hybridization (FISH) is a process which vividly paints chromosomes or portions of chromosomes with fluorescent molecules. This technique is useful for identifying chromosomal abnormalities and gene mapping. Less

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Subject:
Education
Life Science
Mathematics
Material Type:
Images and Illustrations
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
National Health Museum
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Access Excellence @ The National Health Museum: The Site for Health and Bioscience Teachers and Learners
Author:
Access Excellence @ the National Health Museum REV
Darryl Leja (Access Excellence @ the National Health Museum REV)
Darryl Leja (National Human Genome Research Institute REV)
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A Family in Need: In-Class Case Study on Cancer Genetics
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“A Family in Need” was designed as an in-class problem-based learning activity ... More

“A Family in Need” was designed as an in-class problem-based learning activity for students to learn about several innovative medical applications of molecular biology. Students assume the role of a second-year medical student assigned to work with a pediatric oncologist who has just biopsied a tumor-like growth in the adrenal gland of her 17-year-old patient, Lee F. After taking Lee’s family history and performing a pedigree analysis, students review clinical and genetic characteristics of several syndromes associated with adrenal cancer. Students then explore various diagnostic and biomedical research techniques such as PCR, DNA sequencing, and pre-implantation genetic diagnosis. The case concludes with a consideration of how to treat Lee’s condition with the help of gene cloning and the potential of gene therapy. Although originally written for an upper-level college genetics course, the case could also be adapted for an introductory molecular/cellular biology course, a non-majors biology course, or a professional school medical genetics course. Less

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Subject:
Genetics
Material Type:
Case Study
Lecture Notes
Unit of Study
Provider:
National Center for Case Study Teaching in Science
Provider Set:
Case Study Teaching in Science
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Fields, Forces and Flows in Biological Systems, Spring 2007
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This course introduces the basic driving forces for electric current, fluid flow, ... More

This course introduces the basic driving forces for electric current, fluid flow, and mass transport, plus their application to a variety of biological systems. Basic mathematical and engineering tools will be introduced, in the context of biology and physiology. Various electrokinetic phenomena are also considered as an example of coupled nature of chemical-electro-mechanical driving forces. Applications include transport in biological tissues and across membranes, manipulation of cells and biomolecules, and microfluidics. Less

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Subject:
Biology
Material Type:
Full Course
Homework and Assignments
Lecture Notes
Syllabi
Provider:
M.I.T.
Provider Set:
MIT OpenCourseWare
Author:
Han, Jongyoon (Jay)
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Forensic Biochemistry: DNA Testing of Plant Material
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Samples of soil were recovered from an Indiana murder victim's car and ... More

Samples of soil were recovered from an Indiana murder victim's car and shoes. This experiment isolates DNA from plants grown from seeds that were found on the shoes. Two different ecotypes of a weed grow in different areas of Indiana. This difference can be detected using the polymerase chain reaction (PCR). DNA is extracted from the seedlings using the REDExtract-N-Amp Plant PCR Kit. Specific regions of the isolated DNA are then amplified by PCR. The PCR products are analyzed by gel electrophoresis and the results used to determine which suspect was in the same area as the victim. With data from previous exercises the students can determine who committed the murder. An alternate CTAB DNA isolation procedure is also included. Less

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Subject:
Biology
Material Type:
Activities and Labs
Lesson Plans
Provider:
Association for Biology Laboratory Education (ABLE)
Author:
Anna Wilson
Clint Chapple
Jo Cusumano
Susan J. Karcher
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Freshman Seminar: Structural Basis of Genetic Material: Nucleic Acids, Fall 2005
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Since the discovery of the structure of the DNA double helix in ... More

Since the discovery of the structure of the DNA double helix in 1953 by Watson and Crick, the information on detailed molecular structures of DNA and RNA, namely, the foundation of genetic material, has expanded rapidly. This discovery is the beginning of the "Big Bang" of molecular biology and biotechnology. In this seminar, students discuss, from a historical perspective and current developments, the importance of pursuing the detailed structural basis of genetic materials. Less

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Subject:
Biology
Genetics
Material Type:
Full Course
Homework and Assignments
Syllabi
Provider:
M.I.T.
Provider Set:
MIT OpenCourseWare
Author:
Zhang, Shuguang
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Functions of DNA  (Interactive Animation)
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DNA, which carries the blueprint of life, is the key performer of ... More

DNA, which carries the blueprint of life, is the key performer of the central dogma. In addition to transmitting hereditary information from one generation to the next by means of replication, the genes of DNA code for protein sequences in all organisms. Less

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Subject:
Engineering
Biology
Genetics
Chemistry
Material Type:
Interactive
Provider:
Indian Institute of Technology Bombay
Provider Set:
Project OSCAR
Author:
Deepa John, Harini Chandra,Prof. Sanjeeva Srivastava
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Fundamentals of Gene Regulation  (Interactive Animation)
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The DNA of different tissues and cell types is the same in ... More

The DNA of different tissues and cell types is the same in a specific organism unlike the RNA and protein content. Gene regulation must therefore operate to produce different amounts of different RNA in different cell types, from the same DNA. Less

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Subject:
Engineering
Biology
Chemistry
Material Type:
Interactive
Provider:
Indian Institute of Technology Bombay
Provider Set:
Project OSCAR
Author:
Rekha Jain, Harini Chandra,Prof. Sanjeeva Srivastava
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Games and Activities to Teach Molecular Genetics
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This series of activities demonstrates to the biology student that the cell ... More

This series of activities demonstrates to the biology student that the cell is not a static object. It is a complex functioning unit carrying on those activities necessary for life. This unit emphasizes the role of nucleic acids (RNA and DNA) and cell organelles within the cell as interrelated cooperating and functioning components dependent upon one another for the survival of the whole. Less

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Subject:
Education
Life Science
Genetics
Material Type:
Activities and Labs
Instructional Material
Teaching and Learning Strategies
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
National Health Museum
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Access Excellence @ The National Health Museum: The Site for Health and Bioscience Teachers and Learners
Author:
Access Excellence @ the National Health Museum REV
Mark Fairbanks (Paso Robles High School REV)
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Gel Electrophoresis
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In this activity, learners simulate the process of DNA fingerprinting by using ... More

In this activity, learners simulate the process of DNA fingerprinting by using electricity to separate colored dyes. Learners use simple materials to assemble a comb (electrophoresis chamber) to hold the samples, make a 0.2% sodium bicarbonate buffer and 1% gel solution, connect a high voltage power supply, and prepare 5 different samples. Then learners test their model and observe each sample. Less

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Subject:
Engineering
Education
Life Science
Ecology
Forestry and Agriculture
Chemistry
Physics
Material Type:
Activities and Labs
Data
Instructional Material
Lesson Plans
Simulations
Provider:
Science and Math Informal Learning Educators (SMILE)
Provider Set:
SMILE Pathway: Science and Math Activities in One Search
Author:
Julie Yu
National Science Foundation
The Exploratorium
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Gene
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The functional and physical unit of heredity passed from parent to offspring. ... More

The functional and physical unit of heredity passed from parent to offspring. Genes are pieces of DNA, and most genes contain the information for making a specific protein. Less

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Subject:
Education
Life Science
Mathematics
Material Type:
Images and Illustrations
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
National Health Museum
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Access Excellence @ The National Health Museum: The Site for Health and Bioscience Teachers and Learners
Author:
Access Excellence @ the National Health Museum REV
Darryl Leja (National Human Genome Research Institute REV)
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Gene - A More Detailed Description
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This detailed view shows relationships of the base pairs of DNA, introns ... More

This detailed view shows relationships of the base pairs of DNA, introns and exons, gene and chromosome. Less

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Subject:
Education
Life Science
Mathematics
Material Type:
Images and Illustrations
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
National Health Museum
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Access Excellence @ The National Health Museum: The Site for Health and Bioscience Teachers and Learners
Author:
Access Excellence @ the National Health Museum REV
Darryl Leja (Access Excellence @ the National Health Museum REV)
Darryl Leja (National Human Genome Research Institute REV)
Less
Gene Expression During Development: Experimental Design Problem
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This problem challenges students to design experiments using techniques measuring gene expression ... More

This problem challenges students to design experiments using techniques measuring gene expression (reverse transcriptase PCR, microarrays, in situ hybridization). Less

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Subject:
Education
Life Science
Genetics
Material Type:
Activities and Labs
Instructional Material
Provider:
Science Education Resource Center (SERC)
SERC at Carlton College
Provider Set:
Pedagogy in Action
Starting Point (SERC)
Author:
Debby Walser-Kuntz
Sarah Deel
Susan Singer
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General Biology I
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An integrated course stressing the principles of biology. Life processes are examined ... More

An integrated course stressing the principles of biology. Life processes are examined primarily at the molecular and cellular levels. Intended for students majoring in biology or for non-majors who wish to take advanced biology courses. Less

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Subject:
Biology
Genetics
Chemistry
Material Type:
Activities and Labs
Full Course
Lecture Notes
Syllabi
Provider:
UMass Boston
Provider Set:
UMass Boston OpenCourseWare
Author:
Ph.D.
Professor Brian White
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Genes
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Illustration of the placement of genes in a chromosome. A gene can ... More

Illustration of the placement of genes in a chromosome. A gene can be defined as a region of DNA that controls a hereditary characteristic. It usually corresponds to a sequence used in the production of a specific protein or RNA. A gene carries biological information in a form that must be copied and transmitted from each cell to all its progeny. This includes the entire functional unit: coding DNA sequences, non-coding regulatory DNA sequences, and introns. Genes can be as short as 1000 base pairs or as long as several hundred thousand base pairs. It can even be carried by more than one chromosome. The estimate for the number of genes in humans has decreased as our knowledge has increased. As of 2001, humans are thought to have between 30,000 and 40,000 genes. Less

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Subject:
Education
Life Science
Mathematics
Material Type:
Images and Illustrations
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
National Health Museum
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Access Excellence @ The National Health Museum: The Site for Health and Bioscience Teachers and Learners
Author:
Access Excellence
Access Excellence @ the National Health Museum REV
Permissions Manager (Access Excellence @ the National Health Museum REV)
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Genetic Control of Cell Chemistry Using Serratia marcescens
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This exercise allows students to investigate the genetic control of a biosynthetic ... More

This exercise allows students to investigate the genetic control of a biosynthetic pathway: the production of the red pigment prodigiosin by the bacterium Serratia marcescens. The students perform feeding trials with mutant strains to examine the genetic control of this biosynthetic pathway. Auxotrophic strains of the bacteria which are blocked at different places in the pathway exhibit different colors, the wild-type is a deep red color. Pairwise feeding trials enable the students to determine which strains are blocked at which point in the pathway. Students will then use an explanatory system to determine the pathway's shape and learn about its genetic control. Less

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Subject:
Biology
Material Type:
Activities and Labs
Lesson Plans
Provider:
Association for Biology Laboratory Education (ABLE)
Author:
Elise Schmidt
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Genetic Drift
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In this biology simulation, students use a mathematical simulation of genetic drift ... More

In this biology simulation, students use a mathematical simulation of genetic drift to answer questions about the factors that influence this evolutionary process. Students run a series of simulations varying allele frequency and population size and then analyze their data and propose a model to explain their results. Less

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Subject:
Education
Life Science
Mathematics
Material Type:
Activities and Labs
Instructional Material
Provider:
Science Education Resource Center (SERC)
Provider Set:
Starting Point: Teaching Entry Level Geoscience
Author:
Scott Cooper
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Genetics Book List
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This OLogy reference list has seven kid-friendly books on genetics. A short ... More

This OLogy reference list has seven kid-friendly books on genetics. A short description is given for each title, along with author name and publisher. The list includes: illustrated looks at cells, genes, and DNA, hands-on activities and solve-it-yourself mysteries, and easy-to-understand and thought-provoking explorations of cloning and genetic engineering. Less

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Subject:
Biology
Genetics
Material Type:
Readings
Provider:
American Museum of Natural History
Provider Set:
American Museum of Natural History
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