This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"It’s a feared moment for every scientist: the discovery that years of painstaking research has led to results that can't be repeated. Many think that poorly characterized antibodies have contributed to this reproducibility crisis more than any other laboratory tool. A new study published in Molecular Cell supports this hypothesis, at least in the context of chromatin immunoprecipitation. Although accurate ChIP interpretation depends on near-perfect antibody specificity, the report shows that many of these reagents are far less capable than their advertising suggests, which calls into question several widely accepted paradigms on genomic regulation. The study focused on histone post-translational modifications; specifically all three methylation states of lysine 4 on histone H3. Through ChIP experiments, H3K4 methylation has been strongly linked to transcriptional control..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

Students construct paper recombinant plasmids to simulate the methods genetic engineers use to create modified bacteria. They learn what role enzymes, DNA and genes play in the modification of organisms. For the particular model they work on, they isolate a mammal insulin gene and combine it with a bacteria's gene sequence (plasmid DNA) for production of the protein insulin.

Biology is designed for multi-semester biology courses for science majors. It is grounded on an evolutionary basis and includes exciting features that highlight careers in the biological sciences and everyday applications of the concepts at hand. To meet the needs of today’s instructors and students, some content has been strategically condensed while maintaining the overall scope and coverage of traditional texts for this course. Instructors can customize the book, adapting it to the approach that works best in their classroom. Biology also includes an innovative art program that incorporates critical thinking and clicker questions to help students understand—and apply—key concepts.

By the end of this section, you will be able to:Describe the structure of DNAExplain the Sanger method of DNA sequencingDiscuss the similarities and differences between eukaryotic and prokaryotic DNA

By the end of this section, you will be able to:Describe the structure of prokaryotic and eukaryotic genomesDistinguish between chromosomes, genes, and traitsDescribe the mechanisms of chromosome compaction

The goal of this course is to teach both the fundamentals of nuclear cell biology as well as the methodological and experimental approaches upon which they are based. Lectures and class discussions will cover the background and fundamental findings in a particular area of nuclear cell biology. The assigned readings will provide concrete examples of the experimental approaches and logic used to establish these findings. Some examples of topics include genome and systems biology, transcription, and gene expression.

This genomics education lesson plan was formulated and tested on some year 6 students with the help of their teacher Michelle Pardini at the Hong Kong ICS School. Using the example of the ongoing citizen science Bahinia Genome project from Hong Kong it hopes to serve as a model to inspire and inform other national genome projects, and aid the development of crucial genomic literacy and skills across the globe. Inspiring and training a new generation of scientists to use these tools to tackle the biggest threats to mankind: climate change, disease, and food security. It is released under a CC-BY SA 4.0 license, and utilised the following slide deck and final quiz. Promoting open science, all of the data and resources produced from the project is immediately put into the public domain. Please feel free to utilise, adapt and build upon any of these as you wish. The open licence makes these open education resources usable just with attribution and posting of modified resources under a similar manner. Contact BauhiniaGenome if you have any questions or feedback.Bauhinia Genome overviewFor a slidedeck for the lesson plan laid out here you can use the set in slideshare here.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Prokaryotes are assumed to be the oldest existing life form on earth and are found in almost all ecosystems. Genome sequencing can provide a blueprint for the evolutionary and functional diversity of prokaryotes, but what proportion of bacteria and archaea on earth have actually had their genomes sequenced? A new study aimed to explore this basic question. By aligning the sequences released by the Earth Microbiome Project with 155,810 prokaryotic genomes from public databases, researchers found that the median proportions of the genome-sequenced cells and taxa in different biomes reached 38.1% and 18.8%. Due to a set of cosmopolitan OTUs that are found in multiple samples and preferentially sequenced, only 2.1% of the global prokaryotic taxa are represented by sequenced genomes, and most of the biomes were occupied by a few predominant taxa with a high relative abundance, while rarer taxa were less represented..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

For more than two decades J. Craig Venter and his research teams have been pioneers in genomic research. Regarded as one of the leading scientist of the 21st century, Venter discusses how he is applying tools and techniques developed to sequence the human genomes to discover new genes of microbes from around the world. (57 minutes)

This course discusses the principles of genetics with application to the study of biological function at the level of molecules, cells, and multicellular organisms, including humans. The topics include: structure and function of genes, chromosomes and genomes, biological variation resulting from recombination, mutation, and selection, population genetics, use of genetic methods to analyze protein function, gene regulation and inherited disease.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Buried within glaciers is a history of ancient ecosystems, including microbes and viruses past. Some of the airborne microbes and viruses that populate our atmosphere are often carried by snowflakes or dust onto the surface of glaciers. By studying glacial ice cores, scientists can reconstruct histories of microbes and viruses and the climatic and environmental conditions they experienced. Two problems that plague current methods of analyzing ice cores are contamination and low microbe biomass. To address these challenges, researchers recently developed a method for decontaminating ice core surfaces and extracting clean inner ice to study microbes and viruses. When applied to ice from the Guliya ice cap on the Tibetan Plateau in China, the method revealed a unique viral community of mostly novel taxa, providing the first window into viral genomes, communities, and functions in ancient glacier environments..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"The next time you swat away that fly buzzing around your head, consider this: an international team of researchers has shown that common houseflies and blowflies are more than just annoying insects. Covered with hundreds of different bacterial species, they’re also a type of airborne delivery service, transporting pathogens from organic decaying matter right to your countertops, food…and body. Although flies have been long known to spread disease, the researchers show that we’ve previously underestimated both the number and diversity of microbes that each insect can transmit. The team devised a new optimized way to collect flies without cross-contaminating them with other microorganisms and used the method to amass 116 flies from urban, rural, and natural sites on three continents..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Plants are intimately connected to the microbiomes they host. These microbial communities provide plants with nutrients. and protection from environmental stress. While scientists know that plant-derived factors shape the composition of microbiomes. it’s unclear whether host evolution also plays a role. To find out, researchers recently investigated seeds of the genus Oryza, the common rice plant. They examined the effects of speciation and domestication of rice on seed microbiomes. and found that speciation gave rise to distinct communities of bacteria and fungi in seeds. Similarly, domestication tended to produce variations in the composition of fungal communities while conserving bacterial communities. These findings indicate that while evolutionary processes can affect microbiome composition in random fashion. the environmental changes that accompany domestication contribute to the assembly of microbiomes in deterministic ways..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Genomics research has greatly increased understanding of the human gut microbiome, but the existing reference databases remain insufficient, failing to map up to half of the sequences obtained in human gut studies. To solve this problem, researchers recently created HumGut, a comprehensive global reference database for the genomes of gut microbes in healthy humans. The researchers built the database by comparing nearly half a million publicly available prokaryote genomes with over 3,500 gut metagenomes from healthy humans worldwide, and retaining the prokaryote genomes that closely matched the sequences in healthy human guts. HumGut was approximately the same size as the recently released UHGG collection and half the size of a standard reference database. However, HumGut outperformed both other databases in classifying metagenomic reads from human gut samples, resulting in a lower percentage of unclassified reads..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.