This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Microbial life can be found in nearly every environment on earth. These tiny organisms can significantly impact their surroundings, be it deep-sea microbes influencing the ecology of the ocean floor or the human gut microbiome affecting health. Metagenomics, or the analysis of microbial DNA in different environments, has dramatically increased what is known about microbial life. These sequencing-based techniques are not dependent on culturing microbes, which can be an incredibly difficult undertaking. However, isolating and cultivating microbes remains important to both confirm and expand upon those results. Moreover, cultivated microbes could potentially be used as probiotics or biocontrol agents or for industrial purposes. To date, most environmental microbes remain uncultured, but metagenomic data can be leveraged to help researchers isolate and cultivate new ones..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

I ProteoCool is a an open source with simple but Cool Protocols for newbies in the field of molecular biology and protein chemistry.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"This study evaluated the safety, local tolerability, pharmacodynamics and pharmacokinetics of a once-weekly drug candidate versus once-daily growth hormone in children with growth hormone deficiency. This phase 1 trial was conducted in 14 pediatric endocrinology clinics in eight countries. The drug candidate – somapacitan – is a growth hormone derivative that binds reversibly to albumin in the blood, resulting in an extended half-life and prolonged effects. Because of this, the frequency of administration of this medication can be reduced. Somapacitan is injected subcutaneously using a prefilled pen. Clinical trials in adults have suggested the feasibility of a once-weekly dosing regimen of somapacitan based on its PK/PD profile. However, no such study has been conducted for children with growth hormone deficiency. Until now. To evaluate its safety in children, researchers studied 32 boys and girls aged 6 to 13 with a confirmed diagnosis of growth hormone deficiency..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Next-generation sequencing has fostered an explosion of microbiome research. Due to the role our microbiota play in health, there is growing interest in cultivating beneficial bacterial strains for ‘next-generation probiotics’. However, many important species are difficult to cultivate. _Faecalibacterium prausnitzii_ is associated with healthy gut microbiota but is extremely oxygen sensitive, and _Christensenella minuta_ is a health-associated keystone species that is under-represented in the microbial community. A previously described cell sorter modified for anaerobic sorting conditions could be used to isolate sensitive strains of bacteria like this. First, researchers established that reference strains of _F. prausnitzii_ and _C. minuta_ had only minor viability loss during sorting and culture in this system. Then. they generated polyclonal antibodies against _F. prausnitzii _and _C. minuta_..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

Western blots are widely used in molecular cell biology to identify proteins-of-interest in complex protein samples. In western blots, antibodies and detection reagents are used to stain membranes containing replicas of polyacrylamide gels. This module introduces students to the theory and practice of western blots. In this module, students:learn about the biological origins and structural properties of antibodies that underly their specificity.learn how epitope tags allow the detection of proteins-of-interest on western blots.prepare membrane replicas containing protein extracts that have been resolved by SDS-PAGE.analyze protein expression in yeast that have been transformed with expression plasmids.This module is part of an introductory laboratory course, Investigations in Molecular Cell Biology, at Boston College.