Keywords: RNA Interference (13)

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Cell Biology: Structure and Functions of the Nucleus, Spring 2010
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Cell Biology: Structure and Functions of the Nucleus, Spring 2010

The goal of this course is to teach both the fundamentals of ... (more)

The goal of this course is to teach both the fundamentals of nuclear cell biology as well as the methodological and experimental approaches upon which they are based. Lectures and class discussions will cover the background and fundamental findings in a particular area of nuclear cell biology. The assigned readings will provide concrete examples of the experimental approaches and logic used to establish these findings. Some examples of topics include genome and systems biology, transcription, and gene expression. (less)

Subject:
Science and Technology
Material Type:
Homework and Assignments
Readings
Syllabi
Provider:
M.I.T.
Provider Set:
MIT OpenCourseWare
Author:
Sharp, Phillip
Young, Richard
Experimental Molecular Biology: Biotechnology II, Spring 2005
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Experimental Molecular Biology: Biotechnology II, Spring 2005

Laboratory uses yeast as an experimental system to study fundamental problems in ... (more)

Laboratory uses yeast as an experimental system to study fundamental problems in understanding cell cycle and chromosome segregation. Experimental work combines genetic approaches with the tools of molecular and cell biology to identify and characterize novel genes that act on these processes. Instruction and practice in written and oral communication provided. (less)

Subject:
Science and Technology
Material Type:
Activities and Labs
Full Course
Homework and Assignments
Syllabi
Provider:
M.I.T.
Provider Set:
MIT OpenCourseWare
Focus Issue: Drug Discovery
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Focus Issue: Drug Discovery

This week’s issues of Science and Science’s STKE focus on issues related ... (more)

This week’s issues of Science and Science’s STKE focus on issues related to the development of therapeutics, including new methods for identifying drug targets and the goal of personalized medicine based on an individual's genetics. STKE specifically looks at the roles the RNAi can play in the drug target identification process, how understanding the genetics behind disease can lead to new options for therapeutic intervention, and how analysis of the signaling network may provide insight for personalized medicine. (less)

Subject:
Life Science
Material Type:
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Nancy R. Gough (American Association for the Advancement of Science;Science's STKE REV)
Focus Issue: RNA, A Multifunctional Molecule
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Focus Issue: RNA, A Multifunctional Molecule

This week’s issues of Science, Science’s STKE, and SAGE KE (www.sciencemag.org/sciext/rna/) focus ... (more)

This week’s issues of Science, Science’s STKE, and SAGE KE (www.sciencemag.org/sciext/rna/) focus on the increasing complexity that RNA brings to cellular biology. STKE resources include Reviews, Perspectives, and Teaching Resources. STKE specifically looks at the roles for small RNAs in regulation of gene expression and how mRNAs can be selectively activated from cytoplasmic macromolecular structures, RNA granules, to contribute to such processes as synaptic plasticity. (less)

Subject:
Life Science
Material Type:
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Elizabeth M. Adler (American Association for the Advancement of Science;Science's STKE REV)
Nancy R. Gough (American Association for the Advancement of Science;Science's STKE REV)
How Will RNAi Facilitate Drug Development?
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How Will RNAi Facilitate Drug Development?

Development of effective drugs for treatment of human disease relies on identification ... (more)

Development of effective drugs for treatment of human disease relies on identification of therapeutic molecular targets. The identification of targets to treat human disease has previously relied on genetic screens in model organisms, and less robust or lower throughput approaches in mammalian systems. RNA interference (RNAi) makes possible, for the first time, the use of large-scale functional genomics approaches for target identification in human cells. This remarkable breakthrough has the potential to influence every facet of the drug discovery process, and is poised to revolutionize drug development. Reports of RNAi screens for the identification of novel genes implicated in apoptosis, cell division, and drug resistance support the enormous promise of this technology. Here, we discuss the potential impact of RNAi screens on target identification and validation and consider issues that warrant caution when interpreting RNAi screening results. (less)

Subject:
Life Science
Material Type:
Activities and Labs
Images and Illustrations
Instructional Material
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Aimee L. Jackson (Rosetta Inpharmatics LLC. (Merck & Co); REV)
Steven Bartz (Rosetta Inpharmatics LLC. (Merck & Co); REV)
Intracellular Delivery Strategies for MicroRNAs and Potential Therapies for Human Cardiovascular Diseases
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Intracellular Delivery Strategies for MicroRNAs and Potential Therapies for Human Cardiovascular Diseases

MicroRNAs (miRNAs) effectively regulate gene expression in cultured cells and human disease ... (more)

MicroRNAs (miRNAs) effectively regulate gene expression in cultured cells and human disease models, and such regulation can be blocked with antibodies against miRNAs if miRNA-associated adverse effects occur. Promising findings using miRNAs to prevent disease progression in animal studies give hope to patients with disorders caused by dysregulated gene expression, such as cardiovascular diseases. Inflammatory cell infiltration, endothelial cell dysfunction, and angiogenesis are common pathologies of cardiovascular diseases. Accumulating data suggest that miRNA-mediated inhibition of gene expression can drive these pathologies in cardiac tissue or vasculature. It is often desirable to deliver exogenously prepared miRNAs or antibodies against miRNAs to target genes or miRNAs in specific cell or tissue types. Because naked miRNAs or antibodies against miRNAs are often unstable in the circulation, investigation has focused on their packaging and efficient delivery to diseased organs. (less)

Subject:
Life Science
Material Type:
Activities and Labs
Images and Illustrations
Instructional Material
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Guo-Ping Shi (Brigham and Women's Hospital and Harvard Medical School;Department of Medicine REV)
Michael A. Shi (Brigham and Women's Hospital and Harvard Medical School;Department of Medicine REV)
Non-coding RNAs: Junk or Critical Regulators in Health and Disease?, Spring 2012
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Non-coding RNAs: Junk or Critical Regulators in Health and Disease?, Spring 2012

Every time we scientists think that we have dissected the precise biological ... (more)

Every time we scientists think that we have dissected the precise biological nature of a process, an incidental finding, a brilliantly designed experiment, or an unexpected result can turn our world upside down. Until recently thought by many to be cellular "junk" because they do not encode proteins, non-coding RNAs are gaining a growing recognition for their roles in the regulation of a wide scope of processes, ranging from embryogenesis and development to cancer and degenerative disorders. The aim of this class is to introduce the diversity of the RNA world, inhabited by microRNAs, lincRNAs, piRNAs, and many others. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literature to discuss and learn about current biological research in a highly interactive setting. Many instructors of the Advanced Undergraduate Seminars are postdoctoral scientists with a strong interest in teaching. (less)

Subject:
Science and Technology
Material Type:
Full Course
Homework and Assignments
Lecture Notes
Readings
Syllabi
Provider:
M.I.T.
Provider Set:
MIT OpenCourseWare
Author:
Nadya Dimitrova
Thales Papagiannakopoulos
RNA Interference: A New Tool for Genetic Analysis and Therapeutics, Fall 2004
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RNA Interference: A New Tool for Genetic Analysis and Therapeutics, Fall 2004

Seminar covering topics of current interest in biology. Includes reading and analysis ... (more)

Seminar covering topics of current interest in biology. Includes reading and analysis of research papers and student presentations. Contact Biology Education Office for topics. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literature to discuss and learn about current biological research in a highly interactive setting. To understand and treat any disease with a genetic basis or predisposition, scientists and clinicians need effective ways of manipulating the levels of genes and gene products. Conventional methods for the genetic modification of many experimental organisms are technically demanding and time consuming. Just over 5 years ago, a new mechanism of gene-silencing, termed RNA interference (RNAi), was discovered. In addition to being a fascinating biological process, RNAi provides a revolutionary technology that has already changed the way biomedical research is done and that may even prove useful for genetic interventions in a clinical context. In this course, students learn how RNAi was discovered, how it works, and what its physiological relevance might be. How RNAi can be harnessed to modulate gene expression and perform genetic screens, both in cells and in various organisms is also covered. Finally, this course examines the first attempts to use RNAi for the treatment of models of human diseases in experimental animals. (less)

Subject:
Science and Technology
Material Type:
Full Course
Homework and Assignments
Syllabi
Provider:
M.I.T.
Provider Set:
MIT OpenCourseWare
Author:
Kissler, Stephan
Ventura, Andrea
Science Signaling Podcast: 25 October 2011
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Science Signaling Podcast: 25 October 2011

This Podcast features a conversation with authors of a Research Resource published ... (more)

This Podcast features a conversation with authors of a Research Resource published in the 25 October 2011 issue of Science Signaling. Although the extracellular signal–regulated kinase (ERK) pathway has been extensively studied, our understanding of all the regulatory interactions that modulate signaling is by no means complete. Adam Friedman and Norbert Perrimon discuss their group’s strategy of combining functional and genomics approaches to identify common and specific regulators of ERK signaling in the fruit fly Drosophila melanogaster. The ERK pathway plays an important role in normal developmental and physiological contexts as well as in disease states, such as tumorigenesis. (less)

Subject:
Life Science
Physics
Education
Material Type:
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Adam A. Friedman (Harvard Medical School;Department of Genetics REV)
Annalisa M. VanHook (American Association for the Advancement of Science;Science Signaling REV)
Norbert Perrimon (Harvard Medical School;Department of Genetics REV)
Therapeutic Potential of Antisense Nucleic Acid Molecules
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Therapeutic Potential of Antisense Nucleic Acid Molecules

Elucidation of many disease-related signal transduction and gene expression pathways has provided ... (more)

Elucidation of many disease-related signal transduction and gene expression pathways has provided unparalleled opportunities for the development of targeted therapeutics. The types of molecules in development are increasingly varied and include small-molecule enzyme inhibitors, humanized antibodies to cell surface receptors, and antisense nucleic acids for silencing the expression of specific genes. This Perspective reviews the basis for various antisense strategies for modulating gene expression, including RNA interference, and discusses the prospects for their clinical use. (less)

Subject:
Life Science
Material Type:
Images and Illustrations
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Alan M. Gewirtz (University of Pennsylvania School of Medicine;Division of Hematology and Oncology REV)
Joanna B. Opalinska (Pommeranian Medical Academy;Department of Hematology REV)
VideoLab: Butting Heads in the Termite Mound
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VideoLab: Butting Heads in the Termite Mound

Butting behavior among workers in a colony of termites (Cryptotermes secundus), which ... (more)

Butting behavior among workers in a colony of termites (Cryptotermes secundus), which increases when queens are removed. Butting is associated with reproductive dominance, and workers that go on to replace the king or queen display more butting than workers that do not change caste. Using the frequency of butting behavior as a behavioral proxy for queen replacement, and combining this with studies of RNA interference, Korb et al. were able to identify the single gene responsible for the reproductive division of labor between queens and workers in this social insect species. (less)

Subject:
Life Science
Education
Material Type:
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Judith Korb (University of Osnabrueck, and University of Regensburg;Behavioral Biology and Biology)
Katharina Hoffmann (University of Osnabrueck;Behavioral Biology)
Kevin R. Foster (Harvard University;Center for Systems Biology)
Michael Rehli (University Hospital Regensburg;Department of Hematology)
Tobias Weil (University of Regensburg, and University Hospital Regensburg;Department of Biology and Hematology)
Viral Modulators of Cullin RING Ubiquitin Ligases: Culling the Host Defense
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Viral Modulators of Cullin RING Ubiquitin Ligases: Culling the Host Defense

Cullin RING ubiquitin ligases (CRULs) are found in all eukaryotes and play ... (more)

Cullin RING ubiquitin ligases (CRULs) are found in all eukaryotes and play an essential role in targeting proteins for ubiquitin-mediated destruction, thus regulating a plethora of cellular processes. Viruses manipulate CRULs by redirecting this destruction machinery to eliminate unwanted host cell proteins, thus allowing viruses to slip past host immune barriers. Depending on the host organism, virus-modified CRULs can perform an amazing range of tasks, including the elimination of crucial signal transduction molecules in the human interferon pathway and suppression of virus-induced gene silencing in plants. This Perspective summarizes recent advances in our understanding of how viral proteins manipulate the function of CRULs. (less)

Subject:
Life Science
Material Type:
Activities and Labs
Data
Images and Illustrations
Instructional Material
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Klaus Fruh (University of Alberta;Department of Medical Microbiology and Immunology REV)
Michele Barry (University of Alberta;Department of Medical Microbiology and Immunology REV)
The phenomenon of RNA interference (RNAi)
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The phenomenon of RNA interference (RNAi)

The phenomenon of RNA interference (RNAi) -- increasingly recognized as a promising ... (more)

The phenomenon of RNA interference (RNAi) -- increasingly recognized as a promising avenue for treating genetic diseases -- is made more accessible in this interactive presentation, which seamlessly combines audio narration, online video clips, a pop-up glossary, and intuitive navigation. (less)

Subject:
Life Science
Education
Material Type:
Images and Illustrations
Instructional Material
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Doug Huff (Arkitek Studios;)
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