Tissue Culture BT375 WSU

Plant regeneration, cloning, transformation, lab techniques and media requirements for plant cell tissue culture or micropropagation.

A.  Historical Plant Regeneration

1.  Video from Cornell University Classical Plant Breeding.  Then and Now
2.  Video from Cornell University Classical Plant Breeding.  Transgenic Plants 
3.  Video from Cornell University Classical Plant Breeding.  Conclusions
4.  Powerpoint lecture from Adam Blalok of Tennessee State University.  Plant propagation
5. Video from Cornell University Classical Plant Breeding. History of plant regeneration.  Plant propagation
6.  Video from Cornell University Classical Plant Breeding.  Propagation Methods 
7. Video from Cornell University Classical Plant Breeding.  Apple production by layering
8. Video from Cornell University Classical Plant Breeding.  Apple Grafting
9. Video from Cornell University Classical Plant Breeding.  Test tube plants
10.  Article: Hussain A, Qarshi I.Q., Nazir H., & Ullah I 2012. Plant Tissue Culture: Current Status and Opportunities. Chap 1 in Recent Advances in Plant in vitro Culture eds. Leva A. & Rinaldi L.M. InTechopen.org.  Plant Tissue Culture Background

B. The Lab, Aseptic Techniques and Reagents

1.  Video from Phyto Technology Labs on media preparation for plant tissue culture.  Media
2.  Video from Phyto Technology Labs the lab and aseptic technique for plant cloning.  Aseptic Technique 
3.  Article: Plant Tissue Culture Media by Saad A.I.M. and Elshahed A.M. (2012) from Plant Media
4. Article: Saad, A.I.M. & Elshahed 2012, Plant Tissue Culture Media, in Recent Advances in Plant in vitro Culture  eds. Leva A. and Rinaldi L.M. InTech, DOI http://dx.doi.org/10.5772/50569.  Plant Tissue Culture Media

C.  Micropropagation in detail

1.  Video ppt lecture from Alex Lee on micropropagation.  Micropropagation
2.  Video ppt lecture from Dr. Michael Parkinson, Dublin City University.  Video Lecture PPT