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In this module, students use a simple method to transform the budding yeast, Saccharomyces cerevisiae, with yeast expression plasmids containing the GAL1 promoter. At the end of this module, students will be able to:explain the processes of transformation and complmentation at the molecular leveldesign a selection strategy to isolate transformed strains explain how plasmid-encoded genes can complement gene deficienciesuse replica plating on selective media to identify transformed strains expressing plasmid-encoded genesThis module is part of a semester-long introductory laboratory course, Investigations in Molecular Cell Biology, at Boston College.