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Microbiology Laboratory Exercises, Second Edition

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TABLE OF CONTENTS Introduction Schedule Best Laboratory Practices Working in a Microbiology Laboratory Exercises Preparation of a Culture Medium Culturing and Handling Microorganisms Isolation of a Pure Culture Counting Bacterial Populations Controlling Microorganisms Disinfectants Antimicrobial Agents: Susceptibility Testing Hand Washing The Lethal Effects of Ultraviolet Light Selection of Fungi from Air Microscopy Morphology and Staining of Bacteria Microbial Metabolism Enzyme Assay Metabolic Capabilities Microbial Genetics Transformation of Escherichia coli Isolation of Plasmid DNA Restriction Enzyme Digestion of Plasmid DNA and Gel Electrophoresis Appendices A – List of Organisms Used Throughout the Semester B – Vocabulary C – Media D – Intro to Micropipettes E – Microscopy Pocket Guide F – References and Supplemental Materials

Material Type: Activity/Lab

Authors: Ines Rauschenbach, Ramaydalis Keddis

Microbiology Staining Poster Set

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This poster set was designed for display in a microbiology teaching laboratory. The consistent 12 step layout contributes to a cohesive appearance. The color hints on the bottom of each poster shows what students should expect as results from staining. These posters were designed to be printed as 18x24 in, or displayed digitally.

Material Type: Diagram/Illustration

Author: Eric Osborn

Microbiology

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Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology’s art program enhances students’ understanding of concepts through clear and effective illustrations, diagrams, and photographs.

Material Type: Textbook

Authors: Anh-Hue Thi Tu, Ann Auman, Ann Paterson, Ben Rowley, Brian M. Forster, Clifton Franklund, George Pinchuk, Graciela Brelles-Mariño, Mark Schneegurt, Mark Sutherland, Myriam Alhadeff Feldman, Nina Parker, Paul Flowers, Philip Lister, Summer Allen

Introduction to Environmental Science

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This course uses the basic principles of biology and earth science as a context for understanding environmental policies and resource management practices. Our planet is facing unprecedented environmental challenges, from oil spills to global climate change. In ENSC 1000, you will learn about the science behind these problems; preparing you to make an informed, invaluable contribution to Earth’s future. I hope that each of you is engaged by the material presented and participates fully in the search for, acquisition of, and sharing of information within our class.

Material Type: Textbook

Authors: Alison VandeVoort, Caralyn Zehnder, Christine Mutiti, Donna Bennett, Kalina Manoylov, Samuel Mutiti