This online handbook features a downloadable PDF of pre-and post-visit activities for the Squid Lab, a traveling outreach program hosted by the National Aquarium in Baltimore. The hands-on activities and student sheets may be incorporated into any life science classroom. The Squid Lab begins with an overview of the cephalopods, with a particular emphasis on squid. The overall anatomy of the squid is also discussed to provide students with a preview of what they will see when the dissection begins. Students will learn about the adaptations, defense mechanisms, feeding habits, predators and reproductive strategies of squid. Step by step directions are given to guide the students through the dissection.