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Using Bromelain in Pineapple Juice to Investigate Enzyme Function
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This investigation introduces students to the structure and function of the proteolytic ... More

This investigation introduces students to the structure and function of the proteolytic enzyme, bromelain, which is present in large quantities in the fruit, leaves, and stems of pineapple. In this lab exercise, students study the rate at which bromelain catalyzes the hydrolysis of gelatin (substrate) at different temperatures and pHs. Strips of developed black and white photographic film are placed in freshly squeezed pineapple juice under different experimental conditions. The time it takes for the film to clear is used as an indicator of the rate at which bromelain catalyzes the hydrolysis of the substrate (gelatin), which binds the black silver grains to the plastic backing of the film. This lab exercise has been used in traditional non-majors and mixed majors/non-majors General Biology labs and can easily be adapted to an investigative approach. Less

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Subject:
Biology
Material Type:
Activities and Labs
Lesson Plans
Provider:
Association for Biology Laboratory Education (ABLE)
Author:
Mark Hargrove
William Glider
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.)310.1( tcejbus ngised enotspac eht dna )150.1 ,140.1 ,130.1( stcejbus ngised aera ... More

.)310.1( tcejbus ngised enotspac eht dna )150.1 ,140.1 ,130.1( stcejbus ngised aera ytlaiceps tneuqesbus eht ni desu si hcihw decudortni si esac ngised egral A .naps efil detcepxe dna ,srotcaf laicos dna cimonoce ,tnemnorivne larutan ,tnemnorivne tliub gnitsixe eht fo noitaredisnoc sa llew sa sehcaorppa lacinhcet snrecnoc ylticilpxe ngised tcejorP .)sdaor dna segdirb ,sgnidliub ,.g.e( seitilicaf tliub no sisahpme na htiw ,sesac ngised lareves sedulcnI .gnireenigne livic ni secitcarp dna seussi ngised sa llew sa ,gnivlos-melborp evitaerc dna ngised gnireenigne fo seuqinhcet dna ,sloot ,yroeht eht ot stneduts secudortnI Less

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