We now know how to analyze pure compounds, but what if we have a mixture? Spectrophometry becomes quite complex when dealing with multiple species of compounds at once. In order to purify a compound we can separate if from a mixture based on its intrinsic chemical properties. Remember that fluorescein is negatively charged at a pH above pKa of the carboxyl group. We can take advantage of this fact and use its attraction to positive charges to separate it from other molecules. In ion-exchange chromatography, we will use a stationary phase with a positive charge, allowing negatively charged molecules to bind and positively charged species to flow through. We can then disrupt this interaction and retrieve our now-purified molecule, and use spectrophotometric analysis of our purified fractions to determine how well we were able to separate our molecules.
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Water Treatment Plant Operation Processes II builds off the knowledge and information ...
Water Treatment Plant Operation Processes II builds off the knowledge and information from Water Treatment Plant Operation Processes I This text further covers the subjects of Chemical Feed Calculation in Water Treatment Processes, Contact Time Calculations, Disinfection By-Products and Alternative Disinfectants, Chloramination, Fluoridation, Corrosion, Iron and Manganese, Lime Softening, Ion Exchange, Activated Carbon, Membrane Filtration, UV Disinfection and Scada Operation.