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Ion-Exchange Chromatography
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We now know how to analyze pure compounds, but what if we have a mixture? Spectrophometry becomes quite complex when dealing with multiple species of compounds at once. In order to purify a compound we can separate if from a mixture based on its intrinsic chemical properties. Remember that fluorescein is negatively charged at a pH above pKa of the carboxyl group. We can take advantage of this fact and use its attraction to positive charges to separate it from other molecules. In ion-exchange chromatography, we will use a stationary phase with a positive charge, allowing negatively charged molecules to bind and positively charged species to flow through. We can then disrupt this interaction and retrieve our now-purified molecule, and use spectrophotometric analysis of our purified fractions to determine how well we were able to separate our molecules.

Material Type:
Activity/Lab
Lecture Notes
Student Guide
Textbook
Author:
Cody Taylor
Date Added:
01/28/2016
Water Treatment Plant Operation Processes II
Conditions of Use:
No Strings Attached
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Water Treatment Plant Operation Processes II builds off the knowledge and information from Water Treatment Plant Operation Processes I This text further covers the subjects of Chemical Feed Calculation in Water Treatment Processes, Contact Time Calculations, Disinfection By-Products and Alternative Disinfectants, Chloramination, Fluoridation, Corrosion, Iron and Manganese, Lime Softening, Ion Exchange, Activated Carbon, Membrane Filtration, UV Disinfection and Scada Operation.

Subject:
Environmental Science
Career and Technical Education
Environmental Studies
Mathematics
Chemistry
Hydrology
Material Type:
Textbook
Author:
College of the Canyons
John Rowe
Date Added:
03/20/2020