Updating search results...

Search Resources

2 Results

View
Selected filters:
  • ion-exchange
Ion-Exchange Chromatography
Conditional Remix & Share Permitted
CC BY-NC-SA
Rating
0.0 stars

We now know how to analyze pure compounds, but what if we have a mixture? Spectrophometry becomes quite complex when dealing with multiple species of compounds at once. In order to purify a compound we can separate if from a mixture based on its intrinsic chemical properties. Remember that fluorescein is negatively charged at a pH above pKa of the carboxyl group. We can take advantage of this fact and use its attraction to positive charges to separate it from other molecules. In ion-exchange chromatography, we will use a stationary phase with a positive charge, allowing negatively charged molecules to bind and positively charged species to flow through. We can then disrupt this interaction and retrieve our now-purified molecule, and use spectrophotometric analysis of our purified fractions to determine how well we were able to separate our molecules.

Material Type:
Activity/Lab
Lecture Notes
Student Guide
Textbook
Date Added:
02/06/2015