Search Results (1)

Save

Please log in to save materials.

View
Selected filters:
  • FACS
Directed Evolution: Engineering Biocatalysts, Spring 2008
Conditions of Use:
Remix and Share
Rating

Enzymes, nature's catalysts, are remarkable biomolecules capable of extraordinary specificity and selectivity. ... More

Enzymes, nature's catalysts, are remarkable biomolecules capable of extraordinary specificity and selectivity. Directed evolution has been used to produce enzymes with many unique properties, including altered substrate specificity, thermal stability, organic solvent resistance, and enantioselectivity--selectivity of one stereoisomer over another. The technique of directed evolution comprises two essential steps: mutagenesis of the gene encoding the enzyme to produce a library of variants, and selection of a particular variant based on its desirable catalytic properties. In this course we will examine what kinds of enzymes are worth evolving and the strategies used for library generation and enzyme selection. We will focus on those enzymes that are used in the synthesis of drugs and in biotechnological applications. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literature to discuss and learn about current biological research in a highly interactive setting. Many instructors of the Advanced Undergraduate Seminars are postdoctoral scientists with a strong interest in teaching. Less

More
Subject:
Biology
Material Type:
Full Course
Homework and Assignments
Lecture Notes
Syllabi
Provider:
M.I.T.
Provider Set:
MIT OpenCourseWare
Author:
Love, Kerry
Less
2002 gnirpS ,ngiseD gnireenignE liviC ot noitcudortnI
Rating

.)310.1( tcejbus ngised enotspac eht dna )150.1 ,140.1 ,130.1( stcejbus ngised aera ... More

.)310.1( tcejbus ngised enotspac eht dna )150.1 ,140.1 ,130.1( stcejbus ngised aera ytlaiceps tneuqesbus eht ni desu si hcihw decudortni si esac ngised egral A .naps efil detcepxe dna ,srotcaf laicos dna cimonoce ,tnemnorivne larutan ,tnemnorivne tliub gnitsixe eht fo noitaredisnoc sa llew sa sehcaorppa lacinhcet snrecnoc ylticilpxe ngised tcejorP .)sdaor dna segdirb ,sgnidliub ,.g.e( seitilicaf tliub no sisahpme na htiw ,sesac ngised lareves sedulcnI .gnireenigne livic ni secitcarp dna seussi ngised sa llew sa ,gnivlos-melborp evitaerc dna ngised gnireenigne fo seuqinhcet dna ,sloot ,yroeht eht ot stneduts secudortnI Less

More
Less