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  • Molecular Biology
Do You Really Know What You're Eating?: A Case Study on Genetically Modified Foods
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Starting from a fictional "news" report about an apparent allergic reaction to ... More

Starting from a fictional "news" report about an apparent allergic reaction to a taco tainted by genetically modified corn, students consider some of the techniques and procedures used in modern molecular genetics and microbiology as well as some of the issues associated with genetically modified organisms (GMOs). Originally designed for role-play and PowerPoint assignments, suggestions for a shortened version are also provided. Suitable for a general microbiology course, the case could also be used in an introductory molecular biology course with appropriate modifications. Various levels of coverage of the topic of recombinant DNA are possible. Less

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Subject:
Education
Life Science
Ecology
Forestry and Agriculture
Material Type:
Case Study
Instructional Material
Provider:
National Center for Case Study Teaching in Science
Provider Set:
Case Study Collection
Author:
Mary Celeste Reese
Wayne Shew
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Examining Genetic Diversity in Disjunct Populations using Random DNA Markers
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Molecular biological techniques are used to study naturally occurring genetic variation, and ... More

Molecular biological techniques are used to study naturally occurring genetic variation, and have greatly facilitated the understanding of problems in many areas of biology. In this lab, we examine two populations of terrestrial isopods in the Family Armadillidiidae using Random Amplification of Polymorphic DNA (RAPD Analysis). RAPDs are robust and generally species-independent. Using this technique, we are able to detect and analyze genetic variation within and between two different populations of isopods. This lab exercise could easily be modified for use in an introductory course for majors or non-majors and would be suitable for any organism with low vagility. Less

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Subject:
Biology
Material Type:
Activities and Labs
Lesson Plans
Provider:
Association for Biology Laboratory Education (ABLE)
Author:
Judith A. Guinan
Robert Sheehy
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Experimental Molecular Neurobiology, Fall 2006
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Designed for students without previous experience in techniques of cellular and molecular ... More

Designed for students without previous experience in techniques of cellular and molecular biology, this class teaches basic experimental techniques in cellular and molecular neurobiology. Experimental approaches covered include tissue culture of neuronal cell lines, dissection and culture of brain cells, DNA manipulation, synaptic protein analysis, immunocytochemistry, and fluorescent microscopy. Less

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Subject:
Biology
Material Type:
Activities and Labs
Full Course
Homework and Assignments
Syllabi
Provider:
M.I.T.
Provider Set:
MIT OpenCourseWare
Author:
Hayashi, Yasunori
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Expression Vectors Used in Project-Oriented Teaching Laboratories Chapter 2
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This chapter describes a multi-week project taught in an upper level cell ... More

This chapter describes a multi-week project taught in an upper level cell and molecular biology laboratory. Isolation of sufficient quantities of rare proteins from biological tissues can be difficult. These native proteins are often required for raising antibodies, and for studying enzymatic or regulatory function. Expression vectors are used to obtain large quantities of proteins. The cDNA encoding a protein is cloned into an expression vector. The fusion protein is over-expressed in E. coli, and large quantities of the recombinant protein are obtained by affinity chromatography. SDS-polyacrylamide gel electrophoresis is used to determine the size of the proteins isolated. Less

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Subject:
Biology
Material Type:
Activities and Labs
Lesson Plans
Provider:
Association for Biology Laboratory Education (ABLE)
Author:
Chris J. Staiger
David J. Asai
Susan J. Karcher
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Extracting DNA
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This lesson for students in grades 9-12 introduces DNA, genes, chromosomes, the ... More

This lesson for students in grades 9-12 introduces DNA, genes, chromosomes, the chemicals that make up DNA. After the basic information, students will do an experiment in which they will separate out DNA from peas. Knowing that DNA can be separated will give them a base of understanding for future lessons in biology, evolution, biotechnology, and health technology. Less

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Subject:
Biology
Genetics
Material Type:
Lesson Plans
Teaching and Learning Strategies
Provider:
AAAS
Provider Set:
Science Netlinks
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A Family in Need: In-Class Case Study on Cancer Genetics
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“A Family in Need” was designed as an in-class problem-based learning activity ... More

“A Family in Need” was designed as an in-class problem-based learning activity for students to learn about several innovative medical applications of molecular biology. Students assume the role of a second-year medical student assigned to work with a pediatric oncologist who has just biopsied a tumor-like growth in the adrenal gland of her 17-year-old patient, Lee F. After taking Lee’s family history and performing a pedigree analysis, students review clinical and genetic characteristics of several syndromes associated with adrenal cancer. Students then explore various diagnostic and biomedical research techniques such as PCR, DNA sequencing, and pre-implantation genetic diagnosis. The case concludes with a consideration of how to treat Lee’s condition with the help of gene cloning and the potential of gene therapy. Although originally written for an upper-level college genetics course, the case could also be adapted for an introductory molecular/cellular biology course, a non-majors biology course, or a professional school medical genetics course. Less

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Subject:
Genetics
Material Type:
Case Study
Lecture Notes
Unit of Study
Provider:
National Center for Case Study Teaching in Science
Provider Set:
Case Study Collection
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Fields, Forces and Flows in Biological Systems, Spring 2007
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This course introduces the basic driving forces for electric current, fluid flow, ... More

This course introduces the basic driving forces for electric current, fluid flow, and mass transport, plus their application to a variety of biological systems. Basic mathematical and engineering tools will be introduced, in the context of biology and physiology. Various electrokinetic phenomena are also considered as an example of coupled nature of chemical-electro-mechanical driving forces. Applications include transport in biological tissues and across membranes, manipulation of cells and biomolecules, and microfluidics. Less

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Subject:
Biology
Material Type:
Full Course
Homework and Assignments
Lecture Notes
Syllabi
Provider:
M.I.T.
Provider Set:
MIT OpenCourseWare
Author:
Han, Jongyoon (Jay)
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Forensic Biochemistry: DNA Testing of Plant Material
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Samples of soil were recovered from an Indiana murder victim's car and ... More

Samples of soil were recovered from an Indiana murder victim's car and shoes. This experiment isolates DNA from plants grown from seeds that were found on the shoes. Two different ecotypes of a weed grow in different areas of Indiana. This difference can be detected using the polymerase chain reaction (PCR). DNA is extracted from the seedlings using the REDExtract-N-Amp Plant PCR Kit. Specific regions of the isolated DNA are then amplified by PCR. The PCR products are analyzed by gel electrophoresis and the results used to determine which suspect was in the same area as the victim. With data from previous exercises the students can determine who committed the murder. An alternate CTAB DNA isolation procedure is also included. Less

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Subject:
Biology
Material Type:
Activities and Labs
Lesson Plans
Provider:
Association for Biology Laboratory Education (ABLE)
Author:
Anna Wilson
Clint Chapple
Jo Cusumano
Susan J. Karcher
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Freshman Seminar: Structural Basis of Genetic Material: Nucleic Acids, Fall 2005
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Since the discovery of the structure of the DNA double helix in ... More

Since the discovery of the structure of the DNA double helix in 1953 by Watson and Crick, the information on detailed molecular structures of DNA and RNA, namely, the foundation of genetic material, has expanded rapidly. This discovery is the beginning of the "Big Bang" of molecular biology and biotechnology. In this seminar, students discuss, from a historical perspective and current developments, the importance of pursuing the detailed structural basis of genetic materials. Less

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Subject:
Biology
Genetics
Material Type:
Full Course
Homework and Assignments
Syllabi
Provider:
M.I.T.
Provider Set:
MIT OpenCourseWare
Author:
Zhang, Shuguang
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Gel Electrophoresis
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In this activity, learners simulate the process of DNA fingerprinting by using ... More

In this activity, learners simulate the process of DNA fingerprinting by using electricity to separate colored dyes. Learners use simple materials to assemble a comb (electrophoresis chamber) to hold the samples, make a 0.2% sodium bicarbonate buffer and 1% gel solution, connect a high voltage power supply, and prepare 5 different samples. Then learners test their model and observe each sample. Less

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Subject:
Engineering
Education
Life Science
Ecology
Forestry and Agriculture
Chemistry
Physics
Material Type:
Activities and Labs
Data
Instructional Material
Lesson Plans
Simulations
Provider:
Science and Math Informal Learning Educators (SMILE)
Provider Set:
SMILE Pathway: Science and Math Activities in One Search
Author:
Julie Yu
National Science Foundation
The Exploratorium
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Gene Expression During Development: Experimental Design Problem
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This problem challenges students to design experiments using techniques measuring gene expression ... More

This problem challenges students to design experiments using techniques measuring gene expression (reverse transcriptase PCR, microarrays, in situ hybridization). Less

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Subject:
Education
Life Science
Genetics
Material Type:
Activities and Labs
Instructional Material
Provider:
Science Education Resource Center (SERC)
SERC at Carlton College
Provider Set:
Pedagogy in Action
Starting Point (SERC)
Author:
Debby Walser-Kuntz
Sarah Deel
Susan Singer
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General Biology I
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An integrated course stressing the principles of biology. Life processes are examined ... More

An integrated course stressing the principles of biology. Life processes are examined primarily at the molecular and cellular levels. Intended for students majoring in biology or for non-majors who wish to take advanced biology courses. Less

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Subject:
Biology
Genetics
Chemistry
Material Type:
Activities and Labs
Full Course
Lecture Notes
Syllabi
Provider:
UMass Boston
Provider Set:
UMass Boston OpenCourseWare
Author:
Ph.D.
Professor Brian White
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Genetic Control of Cell Chemistry Using Serratia marcescens
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This exercise allows students to investigate the genetic control of a biosynthetic ... More

This exercise allows students to investigate the genetic control of a biosynthetic pathway: the production of the red pigment prodigiosin by the bacterium Serratia marcescens. The students perform feeding trials with mutant strains to examine the genetic control of this biosynthetic pathway. Auxotrophic strains of the bacteria which are blocked at different places in the pathway exhibit different colors, the wild-type is a deep red color. Pairwise feeding trials enable the students to determine which strains are blocked at which point in the pathway. Students will then use an explanatory system to determine the pathway's shape and learn about its genetic control. Less

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Subject:
Biology
Material Type:
Activities and Labs
Lesson Plans
Provider:
Association for Biology Laboratory Education (ABLE)
Author:
Elise Schmidt
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Genetic Drift
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In this biology simulation, students use a mathematical simulation of genetic drift ... More

In this biology simulation, students use a mathematical simulation of genetic drift to answer questions about the factors that influence this evolutionary process. Students run a series of simulations varying allele frequency and population size and then analyze their data and propose a model to explain their results. Less

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Subject:
Education
Life Science
Mathematics
Material Type:
Activities and Labs
Instructional Material
Provider:
Science Education Resource Center (SERC)
Provider Set:
Starting Point: Teaching Entry Level Geoscience
Author:
Scott Cooper
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Genetics Book List
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This OLogy reference list has seven kid-friendly books on genetics. A short ... More

This OLogy reference list has seven kid-friendly books on genetics. A short description is given for each title, along with author name and publisher. The list includes: illustrated looks at cells, genes, and DNA, hands-on activities and solve-it-yourself mysteries, and easy-to-understand and thought-provoking explorations of cloning and genetic engineering. Less

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Subject:
Biology
Genetics
Material Type:
Readings
Provider:
American Museum of Natural History
Provider Set:
American Museum of Natural History
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A Genetics Lab in a Very Unusual Place
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This online article, from the museum's Musings newsletter for educators, looks at ... More

This online article, from the museum's Musings newsletter for educators, looks at the fully functional Genetics Laboratory that was part of the exhibition The Genomic Revolution. It discusses: the significance of the recent sequencing of the human genome in a historical perspective the process visitors underwent during their hour-and-a-half visit to the lab, where they extracted, isolated, visualized, and sequenced their DNA the follow-up activities for visitors on the AMNH Web site. Less

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Subject:
Biology
Genetics
Material Type:
Teaching and Learning Strategies
Provider:
American Museum of Natural History
Provider Set:
American Museum of Natural History
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Genomic DNA Isolation From Human Cheek Cells
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In this lab activity students isolate genomic DNA from their cheek cells ... More

In this lab activity students isolate genomic DNA from their cheek cells on the inside of their mouths. Students then remove the DNA from those cheek cells. It shows the DNA is in every cell in the body and can be extracted easily. Students use their DNA necklace which they proudly wear around school the rest of the day. Less

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Subject:
Education
Life Science
Material Type:
Activities and Labs
Assessments
Instructional Material
Lesson Plans
Provider:
Science Education Resource Center (SERC)
SERC at Carlton College
Provider Set:
Pedagogy in Action
Pedagogy in Action (SERC)
Author:
Guthmiller, Jim
Jim Guthmiller
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Gleevec and the Cell Cycle Problem
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Students are given a problem about a relatively new treatment for cancer, ... More

Students are given a problem about a relatively new treatment for cancer, Gleevec, and asked to apply and synthesize what they have learned about cell signaling and the eukaryotic cell cycle to explain why this targeted treatment works to prevent cell division with fewer side effects than traditional chemotherapeutic agents. Less

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Subject:
Education
Life Science
Material Type:
Activities and Labs
Instructional Material
Provider:
Science Education Resource Center (SERC)
SERC at Carlton College
Provider Set:
Pedagogy in Action
Starting Point (SERC)
Author:
Debby Walser-Kuntz
Sarah Deel
Susan Singer
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Golden Rice: An Intimate Debate Case
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In this intimate debate case, students consider whether to support the development ... More

In this intimate debate case, students consider whether to support the development and use of Golden Rice as a means to alleviate vitamin A deficiency in the developing world. Since many of the arguments typically raised against genetically modified organisms (GMOs) do not apply to this particular GM crop, students are forced to analyze the facts rather than rely on what they have heard in the media. Developed for an introductory molecular biology undergraduate course, the case could also be used at more senior levels. Less

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Subject:
Education
Life Science
Ecology
Forestry and Agriculture
Nutrition
Material Type:
Case Study
Instructional Material
Provider:
National Center for Case Study Teaching in Science
Provider Set:
Case Study Collection
Author:
Annie PrudĽË_homme Genereux
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