This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Thrombospondin-1, or TSP-1, is a calcium-binding protein implicated in the development of several diseases, including diabetes, cancer, renal failure, and cardiovascular disease. To understand TSP-1’s role in disease, researchers recently examined the protein’s effects on the calcium dynamics, survival, and deformability of red blood cells. In vitro experiments showed that exposure to recombinant human TSP-1 significantly increased calcium levels in red blood cells. TSP-1 treatment also induced an inward ionic current, suggesting possible calcium influx through non-selective cation channels, and TSP-1 caused the deformation of red blood cells. Further experiments revealed that TSP-1 binding to its receptor CD47 could dictate the lifespan of red blood cells in circulation. Altogether, the findings suggest that TSP-1/CD47 signaling could be one targetable pathway in systemic diseases that attack red blood cells..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Growing scientific evidence suggests that gut fungi play a prominent role in the development of disease. To understand how the body mounts an immune response to certain fungi during disease, researchers developed a flow cytometry method called Fungi-Flow, which measures immune responses according to levels of IgG antibodies. The team measured the immune responses of various individuals to 17 commensal and environmental fungi.vIgG responses to different fungi varied strongly between healthy individuals, and Fungi-Flow proved sensitive enough to differentiate immune responses between the sporulated and budding forms of fungi. Experiments probing the composition of fungal communities revealed the presence of two distinct clusters, or ecosystems. Differences between the ecosystems were mainly driven by highly abundant fungi of the genus Saccharomyces but also by less abundant fungi such as Cyberlindnera..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Immune checkpoint blockade has enabled great progress in cancer immunotherapy. TIGIT is a novel immune checkpoint that plays a critical role in cancer immunity. TIGIT’s inhibitory function relies on the molecule’s interaction with the ligand PVR. That makes blocking the TIGIT/PVR interaction a promising therapeutic approach. But small molecules suited for the job have not yet been investigated. Now, research points to the benefits of the molecule liothyronine. Liothyronine is an artificial hormone used to treat hypothyroidism. A combination of molecular docking, screening, and a series of in vitro and in vivo assays revealed that liothyronine could be a suitable TIGIT/PVR blocker. When applied to tumor-bearing mice, liothyronine significantly inhibited tumor growth by enhancing CD8+ T cell infiltration and immune responses..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Next-generation sequencing has fostered an explosion of microbiome research. Due to the role our microbiota play in health, there is growing interest in cultivating beneficial bacterial strains for ‘next-generation probiotics’. However, many important species are difficult to cultivate. _Faecalibacterium prausnitzii_ is associated with healthy gut microbiota but is extremely oxygen sensitive, and _Christensenella minuta_ is a health-associated keystone species that is under-represented in the microbial community. A previously described cell sorter modified for anaerobic sorting conditions could be used to isolate sensitive strains of bacteria like this. First, researchers established that reference strains of _F. prausnitzii_ and _C. minuta_ had only minor viability loss during sorting and culture in this system. Then. they generated polyclonal antibodies against _F. prausnitzii _and _C. minuta_..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Chronic myeloid leukemia (CML) is a rare, spontaneous cancer found in bone marrow. Often the causative mutation generates a fusion protein, BCR-ABL1, with abnormal tyrosine kinase activity, and that abnormal activity is the target of the current tyrosine kinase inhibitor treatments. However, these treatments are expensive if used long term and do not kill cancerous stem cells. Thus, new treatments and treatment targets are needed. One potential category of targets are transcription regulators, such as CDK9 (cyclin-dependent kinase 9). Wogonin is a naturally occurring inhibitor of CDK9, and in a recent study it showed anti-CML effects on cell lines and primary CML cells. Specifically, wogonin induced erythroid differentiation in CML cell lines and primary cells and apoptosis in the KU-812 cell line. Wogonin treatment increased binding between GATA-1 and FOG-1, key players in erythrocyte differentiation and decreased binding between GATA-1 and RUNX1, which regulate megakaryocyte differentiation..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.