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Gene Expression Studies Using Microarrays: Principles, Problems, and Prospects
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Gene Expression Studies Using Microarrays: Principles, Problems, and Prospects

Anumber of mammalian genomes having been sequenced, an important next step is ... (more)

Anumber of mammalian genomes having been sequenced, an important next step is to catalog the expression patterns of all transcription units in health and disease by use of microarrays. Such discovery programs are crucial to our understanding of the gene networks that control developmental, physiological, and pathological processes. However, despite the excitement, the full promise of microarray technology has yet to be realized, as the superficial simplicity of the concept belies considerable problems. Microarray technology is very new; methodologies are still evolving, common standards have yet to be established, and many problems with experimental design and variability have still to be fully understood and overcome. This review will describe the time course of a microarray experiment퉌„RNA isolation from sample, target preparation, hybridization to the microarray probe, data capture, and bioinformatic analysis. For each stage, the advantages and disadvantages of competing techniques are compared, and inherent sources of error are identified and discussed. (less)

Subject:
Life Science
Education
Material Type:
Instructional Material
Lecture Notes
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
PhD David Murphy (University of Bristol Research Centre for Neuroendocrinology)
Human Genome Sequencing Center
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Human Genome Sequencing Center

Web site containing links to human, rat, Drosophilia, cDNA, microbial, bovine, Dictyostelium, ... (more)

Web site containing links to human, rat, Drosophilia, cDNA, microbial, bovine, Dictyostelium, mouse and chimpanzee genome sites (less)

Subject:
Life Science
Social Sciences
Education
Material Type:
Activities and Labs
Data
Instructional Material
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Baylor College of Medicine (Baylor College of Medicine Human Genome Sequencing Center)
Macro-Microarray
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Macro-Microarray

In this activity, learners explore the "nuts and bolts" of gene chips. ... (more)

In this activity, learners explore the "nuts and bolts" of gene chips. Learners construct a simple model of a DNA microarray (also known as gene chips) and learn how microarrays can be used to identify and treat disease--including cancer. This resource includes references and an explanation of microarrays. (less)

Subject:
Science and Technology
Engineering
Life Science
Physics
Technology
Social Sciences
Education
Material Type:
Activities and Labs
Data
Instructional Material
Lesson Plans
Provider:
Exploratorium
Science and Math Informal Learning Educators (SMILE)
Provider Set:
SMILE Pathway
SMILE Pathway: Science and Math Activities in One Search
Author:
Julie Yu
National Science Foundation
The Exploratorium
Microarray Studies of Schizophrenia
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Microarray Studies of Schizophrenia

Professor David Lewis outlines how microarrays have transformed the search for schizophrenia ... (more)

Professor David Lewis outlines how microarrays have transformed the search for schizophrenia genes and led to his group's discovery of the candidate gene, RGS4. (less)

Subject:
Computing and Information
Life Science
Physics
Social Sciences
Material Type:
Instructional Material
Video Lectures
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Amy Nisselle REV
Caren Gough TITLE
Danielle Sixsmith NOTE;ENCODING=QUOTED-PRINTABLE
David Micklos (Cold Spring Harbor Laboratory;DNA Learning Center X-AUDIENCE)
John Connolly (Cold Spring Harbor Laboratory;DNA Learning Center X-AUDIENCE)
Laura Maitland (Mepham High School TITLE)
Mary Colvard TITLE
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2002 llaF ,gnivloS melborP gnireenignE dna sretupmoC ot noitcudortnI

.desu si egaugnal gnimmargorp avaJ ehT .gninnalp dna ,tnemeganam ,ecneics ,gnireenigne ni ... (more)

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