Keywords: Arabidopsis (28)

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Anthocyanin Levels in Arabidopsis
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Anthocyanin Levels in Arabidopsis

This pdf describes a simple protocol that can be used to measure ... (more)

This pdf describes a simple protocol that can be used to measure anthocyanin levels in Arabidopsis and other plant species. There are photographs available in the pdf to show representative plants from each experimental condition. (less)

Subject:
Mathematics
Ecology
Forestry and Agriculture
Geoscience
Life Science
Physics
Social Sciences
Education
Material Type:
Activities and Labs
Images and Illustrations
Instructional Material
Lesson Plans
Student Guide
Teaching and Learning Strategies
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
John Kowalski (Roanoke Valley Governor퉌Ís School for Science and Technology)
Arabidopsis: The Model Plant
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Arabidopsis: The Model Plant

A website that describes the significance of decoding the genome of the ... (more)

A website that describes the significance of decoding the genome of the Arabidopsis plant. Describes why scientists chose this particular plant and what this means for the future. (less)

Subject:
Mathematics
Life Science
Education
Material Type:
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
National Science Foundation (National Science Foundation Undergraduate Education Science, Math, Engineering and Technology)
Blue Plants: Transgenic Plants with the GUS Reporter Gene
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Blue Plants: Transgenic Plants with the GUS Reporter Gene

An investigative laboratory developed for the introductory biology curriculum using transgenic plants ... (more)

An investigative laboratory developed for the introductory biology curriculum using transgenic plants is presented in this chapter. The transgenic Arabidopsis plants we use contain the GUS reporter gene under the control of the cor15a gene promoter, which responds to cold stress. Following induction by cold or other environmental signals, the gusA gene will respond by producing the enzyme beta-glucuronidase (GUS). When plant tissue is incubated with the chromogenic substrate X-gluc, those tissues that produce GUS turn blue. Using investigative experiments, students monitor both the physiological response of plants to these signals, as well as the induction of gene activity as reflected by GUS activity. The GUS assay is highly visible, safe for the undergraduate laboratory, easy to conduct, and relatively inexpensive. Blue Plants, developed at Purdue University with support from NSF-DUE grant #9354721, are one of the Research Link 2000 systems (http://www.researchlink.ferris.edu). (less)

Subject:
Science and Technology
Material Type:
Activities and Labs
Lesson Plans
Provider:
Purdue University
Provider Set:
Association for Biology Laboratory Education (ABLE)
Author:
Susan J. Karcher
Combining Genetics and Cell Biology to Crack the Code of Plant Cell Calcium Signaling
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Combining Genetics and Cell Biology to Crack the Code of Plant Cell Calcium Signaling

Plant hormones and light signals enable plants to respond to changes in ... (more)

Plant hormones and light signals enable plants to respond to changes in their environment. These stimuli are perceived by receptors that transmit responses to the inside of cells by inducing changes in cytoplasmic concentrations of chemical messengers, such as calcium. Many plant hormone and light signals cause elevations in the cytoplasmic calcium concentration. Recent studies are reviewed here that reveal how genetic mutations disrupt stimulus-induced calcium elevations in plant cells. These studies provide genetic evidence for the importance of calcium as a second messenger in plant signal transduction. A classical question has remained unanswered until recently, namely, how can different stimuli use the same second messenger, calcium, to mediate different responses in plants? Recent research and models are reviewed here that show several important mechanisms contributing to specificity in calcium signaling in plant cells. These mechanisms include (i) the activation of different calcium channels in the plasma membrane and organellar membranes of plant cells; (ii) the specific frequencies needed by calcium oscillations to mediate specific responses; (iii) the differential responses of different cell types to the same stimulus; and (iv) within each cell, the importance of intracellular localization of calcium gradients and calcium elevations to elicit the response. These advances show that plant cells contain a sophisticated apparatus that allows them to respond to many different environmental conditions through the second messenger, calcium. (less)

Subject:
Mathematics
Life Science
Material Type:
Activities and Labs
Images and Illustrations
Instructional Material
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Gethyn J. Allen (San Diego;University of California REV)
Julian I. Schroeder (San Diego;University of California REV)
The Control of Flowering in Time and Space
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The Control of Flowering in Time and Space

This journal article provides information about photoperiod controls and its impact on ... (more)

This journal article provides information about photoperiod controls and its impact on flowering. (less)

Subject:
Mathematics
Ecology
Forestry and Agriculture
Geoscience
Life Science
Physics
Social Sciences
Education
Material Type:
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Alexander Graf (John Innes Centre)
Katja E Jaeger (John Innes Centre)
Philip Wigge (John Innes Centre Department of Cell and Developmental Biology)
Cryptochromes: Blue Light Receptors for Plants and Animals
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Cryptochromes: Blue Light Receptors for Plants and Animals

Cryptochromes are blue, ultraviolet-A photoreceptors. They were first characterized for Arabidopsis and ... (more)

Cryptochromes are blue, ultraviolet-A photoreceptors. They were first characterized for Arabidopsis and are also found in ferns and algae; they appear to be ubiquitous in the plant kingdom. They are flavoproteins similar in sequence to photolyases, their presumptive evolutionary ancestors. Sequence comparison indicates that the plant and animal cryptochrome families have distinct evolutionary histories, with the plant cryptochromes being of ancient evolutionary origin and the animal cryptochromes having evolved relatively recently. This process of repeated evolution may have coincided with the origin in animals of a modified circadian clock based on the PERIOD, TIMELESS, CLOCK, and CYCLE proteins. (less)

Subject:
Mathematics
Life Science
Material Type:
Activities and Labs
Images and Illustrations
Instructional Material
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Anthony R. Cashmore (University of Pennsylvania;Plant Science Institute, Department of Biology)
Dongmei Liu (University of Pennsylvania;Plant Science Institute, Department of Biology)
Jose A. Jarillo (University of Pennsylvania;Plant Science Institute, Department of Biology)
Ying-Jie Wu (University of Pennsylvania;Plant Science Institute, Department of Biology)
The Ethylene Pathway: A Paradigm for Plant Hormone Signaling and Interaction
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The Ethylene Pathway: A Paradigm for Plant Hormone Signaling and Interaction

The gas ethylene functions as a hormone in plants. In this review ... (more)

The gas ethylene functions as a hormone in plants. In this review the ethylene biosynthesis and signaling pathway in the reference plant Arabidopsis thaliana are described. The questions of how the ethylene gas is produced and sensed by the receptor protein, and how the different pathway components function to transduce the signal to the nucleus, will be addressed. We also discuss what is known about the regulation of these processes by other growth regulators and how they contribute to the generation of complex ethylene-mediated responses. (less)

Subject:
Mathematics
Life Science
Material Type:
Activities and Labs
Images and Illustrations
Instructional Material
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Jose M. Alonso (La Jolla;The Salk Institute for Biological Studies REV)
Joseph R. Ecker (La Jolla;The Salk Institute for Biological Studies REV)
Ethylene Signaling Pathway
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Ethylene Signaling Pathway

The structural simplicity of the plant hormone ethylene contrasts with its dramatic ... (more)

The structural simplicity of the plant hormone ethylene contrasts with its dramatic effects in various developmental processes, as well as in the cellular processes that ethylene initiates in response to a diversity of environmental signals. A single well-conserved signaling cascade mediates this broad spectrum of responses. Ethylene is perceived by a family of two-component histidine kinase receptors that become inactivated upon ethylene binding. In the absence of the hormone, the receptors activate CTR1, a negative regulator of ethylene responses. Sequence similarity between CTR1 and the Raf protein kinases implies involvement of a mitogen-activated protein kinase cascade in this signaling pathway. The protein EIN2 acts downstream of CTR1 and the possible kinase cascade. Although the biochemical function of EIN2 is not understood, its critical role is manifested by the complete ethylene insensitivity of EIN2 loss-of-function mutants. Downstream of EIN2, a family of plant-specific EIN3-like transcription factors mediate ethylene responses. The regulation of EIN3 stability by ethylene is accomplished by F-box–containing proteins that participate in the formation of a SKP1/cullin/F-box complex that targets proteins for degradation by the proteasome. A large number of ethylene-regulated genes have been identified, including the APETALA2 domain–containing transcription factor genes ERF1 and EDF1 to 4, which suggests the participation of a transcriptional cascade in the ethylene response. The differential regulation of some components of this complex nuclear cascade by other signaling pathways provides a possible mechanism for interaction and signal integration. As new points of intersection with other pathways and additional participants in the pathway are identified, the Connections Map will be updated to include this new information. (less)

Subject:
Mathematics
Computing and Information
Life Science
Education
Material Type:
Activities and Labs
Data
Images and Illustrations
Instructional Material
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Anna N. Stepanova (North Carolina State University;Department of Genetics REV)
Jose M. Alonso (North Carolina State University;Department of Genetics REV)
Germinating on Petri Dishes
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Germinating on Petri Dishes

This is a pdf of both student and teacher instructions on cold-treating ... (more)

This is a pdf of both student and teacher instructions on cold-treating and plating Arabidopsis seeds. (less)

Subject:
Mathematics
Ecology
Forestry and Agriculture
Geoscience
Life Science
Social Sciences
Education
Material Type:
Activities and Labs
Images and Illustrations
Instructional Material
Lesson Plans
Student Guide
Teaching and Learning Strategies
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
David Lally (Virginia Tech Biochemistry)
Dr. Erin L Dolan (Virginia Tech Biochemistry)
A Hitchhiker's Guide to the Proteasome
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A Hitchhiker's Guide to the Proteasome

Regulated degradation of proteins is essential for viability and is involved in ... (more)

Regulated degradation of proteins is essential for viability and is involved in the control of many signal transduction pathways. von Arnim discusses a new model for how substrates destined for degradation by the 26S proteasome may be presented to the proteasome through a physical interaction between the proteasome and a complex consisting of the substrate and a ubiquitin-ligase. The new model suggests that the SCF (Skp1/cullin/F-box) protein complex may physically associate with the proteasome and that this interaction may be regulated by posttranslational modifications, such as phosphorylation or the covalent attachment of the Nedd8 protein, called neddylation. Additionally, other proteins may compete with the SCF complexes for binding to the proteasome and thus present another layer of regulation for controlling substrate targeting for ubiquitin-mediated degradation. (less)

Subject:
Mathematics
Life Science
Material Type:
Activities and Labs
Images and Illustrations
Instructional Material
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Albrecht G. von Arnim (University of Tennessee;Department of Botany REV)
How Do You Disable a Gene?
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How Do You Disable a Gene?

A video filmed at Virginia Tech about how to disable a gene. ... (more)

A video filmed at Virginia Tech about how to disable a gene. David Lally describes the use of Insertional Mutagenesis. (less)

Subject:
Mathematics
Life Science
Education
Material Type:
Images and Illustrations
Instructional Material
Lecture Notes
Simulations
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
David Lally (Virginia Tech Biochemistry)
Dr. Erin L Dolan (Virginia Tech Biochemistry)
Jasmonate Biochemical Pathway
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Jasmonate Biochemical Pathway

Plants possess an interrelated family of potent fatty acid-derived regulators—the jasmonates. These ... (more)

Plants possess an interrelated family of potent fatty acid-derived regulators—the jasmonates. These compounds, which play roles in both defense and development, are derived from tri-unsaturated fatty acids [α-linolenic acid (18:3) or 7Z,10Z,13Z-hexadecatrienoic acid (16:3)]. The lipoxygenase-catalyzed addition of molecular oxygen to α-linolenic acid initiates jasmonate synthesis by providing a 13-hydroperoxide substrate for the formation of an unstable allene oxide that is then subject to enzyme-guided cyclization to produce 12-oxo-phytodienoic acid (OPDA). OPDA has several fates, including esterification into plastid lipids or transformation into the 12-carbon co-regulator jasmonic acid (JA). JA, the best-characterized member of the family, regulates both male and female fertility (depending on the plant species) and is an important mediator of defense gene expression. JA is itself a substrate for further diverse modifications. Genetic dissection of the pathway is revealing how the different jasmonates modulate different physiological processes. Each new family member that is discovered provides another key to understanding the fine control of gene expression in immune responses, in the initiation and maintenance of long-distance signal transfer in response to wounding, in the regulation of fertility, and in the turnover, inactivation, and sequestration of jasmonates, among other processes. The Jasmonate Biochemical Pathway provides an overview of the growing jasmonate family, and new members will be included in future versions of the Connections Map. (less)

Subject:
Mathematics
Computing and Information
Life Science
Education
Material Type:
Activities and Labs
Data
Images and Illustrations
Instructional Material
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Edward E. Farmer (University of Lausanne;Gene Expression Laboratory and Faculty of Biology and Medicine REV)
Robin Liechti (University of Lausanne;Gene Expression Laboratory and Faculty of Biology and Medicine REV)
Light Signals and Flowering
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Light Signals and Flowering

This journal article provides information about light variables and its impact on ... (more)

This journal article provides information about light variables and its impact on flowering. (less)

Subject:
Mathematics
Ecology
Forestry and Agriculture
Geoscience
Life Science
Physics
Social Sciences
Education
Material Type:
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Brian Thomas (University of Warwick)
Model Organisms and Arabidopsis
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Model Organisms and Arabidopsis

Lesson plan with three parts. First part is to get students thinking ... (more)

Lesson plan with three parts. First part is to get students thinking about the types of organism used by research scientists. The second describes characteristics of a model plant. The third part describes the Arabidopsis as a model plant for research scientists. (less)

Subject:
Mathematics
Ecology
Forestry and Agriculture
Geoscience
Life Science
Social Sciences
Education
Material Type:
Activities and Labs
Assessments
Instructional Material
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Dr. John Kowalski (Roanoke Valley Governor퉌Ís School for Science and Technology)
Model of the TIR1 Pathway for Auxin-Mediated Gene Expression
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Model of the TIR1 Pathway for Auxin-Mediated Gene Expression

Auxin mediates numerous plant responses, some of which have been shown to ... (more)

Auxin mediates numerous plant responses, some of which have been shown to require transcriptional regulation. One auxin response pathway, which depends on the relief of transcriptional repression, is mediated by TIR1 (transport inhibitor response protein 1). TIR1 is an auxin receptor and also a subunit of an SCF-type ubiquitin ligase. In the presence of a low concentration of auxin in the nucleus, members of the Aux/IAA family of transcriptional repressors bind to ARF proteins and inhibit the transcription of specific auxin response genes. Increased nuclear concentrations of auxin promote auxin binding to TIR1, causing the Aux/IAA proteins to associate with TIR1 and leading to their degradation by a proteasome-mediated pathway. This decreases the concentration of Aux/IAA proteins in the nucleus and thereby enables the expression of certain auxin response genes. (less)

Subject:
Mathematics
Life Science
Material Type:
Instructional Material
Lecture Notes
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Marta Laskowski (Oberlin College;Biology Department REV)
Molecular Genetic Studies of the Memory of Winter (Cold and Flowering)
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Molecular Genetic Studies of the Memory of Winter (Cold and Flowering)

This journal article provides information about cold temperature and its impact on ... (more)

This journal article provides information about cold temperature and its impact on flowering. (less)

Subject:
Mathematics
Ecology
Forestry and Agriculture
Geoscience
Life Science
Physics
Social Sciences
Education
Material Type:
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Brian Thomas (University of Warwick)
Dr. Sibum Sung (University of Texas at Austin)
PLANT SCIENCE: Paternal Patterning Cue
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PLANT SCIENCE: Paternal Patterning Cue

Access to the article is free, however registration and sign-in are required. ... (more)

Access to the article is free, however registration and sign-in are required. Over the past decade, several mutations that exert maternal effects on embryogenesis have been described in the model plant Arabidopsis thaliana. Bayer et al. describe the first paternal effect on plant embryogenesis, demonstrating that a temporal cue provided by the sperm cell triggers the events leading to the first asymmetric division of the plant embryo. (less)

Subject:
Mathematics
Life Science
Education
Material Type:
Activities and Labs
Images and Illustrations
Instructional Material
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Ueli Grossniklaus (University of Z퀌_rich;)
Phospholipid Signaling in Plants: Holding On to Phospholipase D
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Phospholipid Signaling in Plants: Holding On to Phospholipase D

Plant cells contain various phospholipase-based signaling pathways. In fact, their repertoire of ... (more)

Plant cells contain various phospholipase-based signaling pathways. In fact, their repertoire of phospholipase D (PLD) molecules far outnumbers those of mammalian and yeast cells. Munnik and Musgrave take a broad look at PLD function in animal, yeast, and plant cells, and suggest that a PLD-based connection between membranes and microtubules is a biological property worth considering across species. (less)

Subject:
Mathematics
Life Science
Material Type:
Activities and Labs
Images and Illustrations
Instructional Material
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Alan Musgrave (University of Amsterdam;Swammerdam Institute for Life Sciences (SILS)and Department of Plant Physiology REV)
Teun Munnik (University of Amsterdam;Swammerdam Institute for Life Sciences (SILS)and Department of Plant Physiology REV)
Phosphorelay and Transcription Control in Cytokinin Signal Transduction
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Phosphorelay and Transcription Control in Cytokinin Signal Transduction

Cytokinins are master regulators of a large number of processes in plant ... (more)

Cytokinins are master regulators of a large number of processes in plant development, which is known to be unusually plastic and adaptive, as well as resilient and perpetual. These characteristics allow plants to respond sensitively and quickly to their environments. Recent studies have demonstrated that cytokinin signaling involves a multistep two-component signaling pathway, resulting in the development of a canonical model of cytokinin signaling that is likely representative in plants. This Viewpoint outlines this general model, focusing on the specific example of Arabidopsis, and introduces the STKE Connections Maps for both the canonical module and the specific Arabidopsis Cytokinin Signaling Pathway. (less)

Subject:
Mathematics
Life Science
Material Type:
Images and Illustrations
Reference
Provider:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Provider Set:
BiosciEdNet (BEN): Digital Library Portal for Teaching and Learning in the Biological Sciences
Author:
Jen Sheen (Harvard Medical School /Massachusetts General Hospital;)
2002 llaF ,gnivloS melborP gnireenignE dna sretupmoC ot noitcudortnI

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.desu si egaugnal gnimmargorp avaJ ehT .gninnalp dna ,tnemeganam ,ecneics ,gnireenigne ni ... (more)

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