Biology is designed for multi-semester biology courses for science majors. It is …
Biology is designed for multi-semester biology courses for science majors. It is grounded on an evolutionary basis and includes exciting features that highlight careers in the biological sciences and everyday applications of the concepts at hand. To meet the needs of today’s instructors and students, some content has been strategically condensed while maintaining the overall scope and coverage of traditional texts for this course. Instructors can customize the book, adapting it to the approach that works best in their classroom. Biology also includes an innovative art program that incorporates critical thinking and clicker questions to help students understand—and apply—key concepts.
Western blots are widely used in molecular cell biology to identify proteins-of-interest …
Western blots are widely used in molecular cell biology to identify proteins-of-interest in complex protein samples. In western blots, antibodies and detection reagents are used to stain membranes containing replicas of polyacrylamide gels. This module introduces students to the theory and practice of western blots. In this module, students:learn about the biological origins and structural properties of antibodies that underly their specificity.learn how epitope tags allow the detection of proteins-of-interest on western blots.prepare membrane replicas containing protein extracts that have been resolved by SDS-PAGE.analyze protein expression in yeast that have been transformed with expression plasmids.This module is part of an introductory laboratory course, Investigations in Molecular Cell Biology, at Boston College.
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