This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:
"In recent years, the study of microbial communities has greatly benefitted from advances in both genetic sequencing and more traditional culture-based approaches, unlocking their potential to drive the development of new medical treatments like never before, but the techniques used for sample preservation could affect which microbes are ultimately cultured and thus constrain the discovery of previously undescribed species and microbial functions. To better understand how preservation affects the study of anaerobic bacteria in the gut, researchers froze human fecal samples at -80°C after adding Cary-Blair medium with or without 20% glycerol or 5% dimethyl sulfoxide (DMSO) to some of them. These preservatives are often used when transporting and freezing fecal and other clinical specimens. They found that the preservation conditions affected the number of bacterial anaerobes cultured from the samples, as well as the recovery of particular genera..."
The rest of the transcript, along with a link to the research itself, is available on the resource itself.
- Subject:
- Biology
- Life Science
- Material Type:
- Diagram/Illustration
- Reading
- Provider:
- Research Square
- Provider Set:
- Video Bytes
- Date Added:
- 04/14/2023