This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"A new method for identifying post-translational modifications in proteins promises to cut biomedical researchers’ workload in half. Enabling multiple affinity enrichment procedures to be run in parallel, the one-pot method yields the same search results as traditional methods in less time and from less tissue. As proteomics researchers know well, identifying post-translational modifications in biological samples can be tedious. Enriching samples with target modifications, such as the attachment of acetyl , succinyl or methyl groups to amino acid residues, and matching experimental data with catalogued results involves numerous steps. And the work load is only getting bigger. With exploding interest in how multiple modifications are linked across the vast proteome , the amount of time and the amount of sample required for exploration are skyrocketing in proportion. But with the new one-pot enrichment method, that could soon change..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Acetaminophen (N-acetyl-p-aminophenol, APAP) is a commonly used over-the-counter analgesic and antipyretic, but an overdose can cause severe liver damage or even liver failure, and the therapies for such acetaminophen-induced liver injury (AILI) are limited. To search for new treatment targets, researchers recently investigated the roles of the IL-33/ST2 cytokine signaling pathway in a mouse AILI model. Compared to wild-type mice, IL-33-knockout mice had worse hepatotoxicity after acetaminophen overdose, as indicated by greater hepatocyte necrosis, DNA accumulation, and type I IFN production, suggesting that IL-33 signaling normally has a protective effect. Further investigation in liver cell lines revealed that the binding of IL-33 with its receptor ST2 enhanced the interaction of Beclin-1 with the STING C-terminus..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.

This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:

"Wheat is one of the world’s most important crops. However, wheat production is currently threatened by various fungal diseases throughout the growing season. Fusarium head blight (FHB), caused by _Fusarium graminearum_, is one of the most important fungal diseases of wheat causing serious wheat yield losses, as well as mycotoxin contamination. One approach to combating this type of infection is through autophagy. Autophagy is a highly conserved physiologic process critical for macromolecule turnover, stress response, and survival in eukaryotes and plays an important role in fungal pathogenesis. _Streptomyces hygroscopicus_ S89, a member of the natural wheat microbiota, has shown high efficiency in reducing mycelial growth of _F. graminearum_—possibly through autophagy. Researchers found that the antifungal compound rapamycin secreted by _S. hygroscopicus_ S89 stimulates autophagic flux and affects the acetylome of _F. graminearium_ by promoting the degradation of acetyltransferase Gcn5..."

The rest of the transcript, along with a link to the research itself, is available on the resource itself.