Student Teacher

Description

Overview:
We now know how to analyze pure compounds, but what if we have a mixture? Spectrophometry becomes quite complex when dealing with multiple species of compounds at once. In order to purify a compound we can separate if from a mixture based on its intrinsic chemical properties. Remember that fluorescein is negatively charged at a pH above pKa of the carboxyl group. We can take advantage of this fact and use its attraction to positive charges to separate it from other molecules. In ion-exchange chromatography, we will use a stationary phase with a positive charge, allowing negatively charged molecules to bind and positively charged species to flow through. We can then disrupt this interaction and retrieve our now-purified molecule, and use spectrophotometric analysis of our purified fractions to determine how well we were able to separate our molecules.
Level:
College / Upper Division, Graduate / Professional
Material Type:
Activity/Lab, Lecture Notes, Student Guide, Textbook
Author:
Date Added:
01/28/2016
License:
Creative Commons Attribution-Noncommercial-Share Alike 4.0 Creative Commons Attribution-Noncommercial-Share Alike 4.0
Language:
English
Media Format:
Graphics/Photos, Text/HTML

Comments

*

Standards

No standards aligned yet.

Please this resource to your standards.

Evaluations

No evaluations yet.

Add important feedback and this resource.

Tags (7)