Teaching Western Blots with T antigen and p53

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Author:

Theodore Gurney

Subject:

Science and Technology

Institution Name:

University of Utah

Collection:

Association for Biology Laboratory Education (ABLE)

Grade Level:

Post-secondary

Abstract:

Students perform Western blots with polyacrylamide gel electrophoresis of detergent extracts of SV40-transformed cell lines grown in monolayer culture. The gels are blotted onto nitrocellulose and treated with a monoclonal antibody against SV40 T antigen or against the tumor suppressor p53. Blots are then treated with a secondary antibody (anti-mouse IgG conjugated to horseradish peroxidase), and exposed to revealing chemicals and x-ray film. I give one application: induction of p53 by ultraviolet light.

Course Type:

Learning Module

Languages:

English

Material Type:

Activities and Labs, Lesson Plans

Media Format:

Downloadable docs

Conditions of Use:

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