Polyacrylamide Gel Electrophoresis (PAGE) of Blood Proteins
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- Author:
- John Mordacq, Roberta Ellington
- Subject:
- Science and Technology
- Institution Name:
- Northwestern University
- Collection:
- Association for Biology Laboratory Education (ABLE)
- Grade Level:
- Post-secondary
- Abstract:
In this laboratory exercise, students utilize differential precipitation and polyacrylamide gel electrophoresis in combination with centrifugation and detergent extraction to fractionate the complex mixtures of proteins found in mammalian red blood cells and mammalian blood plasma and to characterize some of the component proteins. Gels are stained with Coomassie blue and migration distances for the unknown proteins are compared with those of known protein standards. Students then determine the sizes of the unknown proteins, compare their relative abundance, speculate about the identities of the components of the various fractions, and evaluate the efficiency of the fractionation techniques.
- Course Type:
- Learning Module
- Languages:
- English
- Material Type:
- Activities and Labs, Lesson Plans
- Media Format:
- Downloadable docs
- Conditions of Use:
-
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