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Molecular Biology

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Subject:
Science and Technology
Institution Name:
The Saylor Foundation
Collection:
Saylor Foundation
Grade Level:
Post-secondary
Abstract:

After a historical introduction to molecular biology, this course describes the basic types of DNA and RNA structure and the molecular interactions that shape them. It describes how DNA is packaged within the cellular nucleus as chromosomes. It also describes the core processes of molecular biology: replication of DNA, transcription of DNA into messenger RNA, and translation of messenger RNA into a protein. These are followed by modifications of these basic processes: regulation of gene expression, DNA mutation and repair, and DNA recombination and transposition. Upon successful completion of this course, students will be able to: discuss the experimental findings that lead to the discovery of inheritance laws; discuss the experimental findings that lead to the identification of DNA as the hereditary material; compare and contrast the structure and function of mRNA, rRNA, tRNA, and DNA; identify the characteristics of catalyzed reactions; compare and contrast enzyme and ribozyme catalyzed reactions; discuss the structure of the chromosome and the consequence of histone modifications in eukaryotes; discuss the stages of transcription, differential splicing, and RNA turnover; predict the translation product of an mRNA using the genetic code; compare and contrast transcription and translation in prokaryotes and eukaryotes; identify codon bias and variations of the standard genetic code; compare and contrast the regulation of prokaryotic and eukaryotic gene expression; predict the activation of an operon and tissue specific gene expression based on the availability of regulators; compare and contrast mutations based on their effect on the gene product; discuss DNA repair mechanisms; discuss DNA recombination, transposition, and the consequence of exon shuffling; design custom-made recombinant DNA using PCR, restriction enzymes, and site-directed mutagenesis; compare and contrast the uses of model organisms; discuss the uses of model organisms in specific molecular biology applications. (Biology 311)

Languages:
English
Material Type:
Assessments, Audio Lectures, Full Course, Homework and Assignments, Lecture Notes, Readings, Syllabi, Textbooks, Video Lectures
Media Format:
Audio, Graphics/Photos, Text/HTML, Downloadable docs, Video
Conditions of Use:
Creative Commons Attribution-Noncommercial 3.0
You are welcome to share, remix, and adapt this course under the terms of the Creative Commons Attribution 3.0 Unported License; however, many linked materials within this course are copyright of their respective authors/owners and may not be openly-licensed. Please respect the copyright and terms of use associated with each resource.
Copyright Holder:
The Saylor Foundation

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