Isolating Multiple Strains of Escherichia coli for Coliphage Isolation, Phage-typing, and Mutant Recovery
Read the Fine Print
- Author:
- Richard Levin
- Subject:
- Science and Technology
- Institution Name:
- Oberlin College
- Collection:
- Association for Biology Laboratory Education (ABLE)
- Grade Level:
- Post-secondary
- Abstract:
Recovery of Escherichia coli from a rectal swab begins a series of procedures combining mastery of technique with productive experiments. After isolating his/her own E. coli strain, each student recovers a unique coliphage from raw sewage and determines its host range, thereby revealing the genetic individuality of each bacterial and viral isolate. Colonies of virus-resistant mutants can often be recovered in regions where sensitive bacteria have been destroyed. After antibiotic sensitivity testing of E. coli isolates, a variety of antibiotic resistant mutants can be recovered, thus yielding genetically marked strains for future bacterial genetics experiments.
- Course Type:
- Learning Module
- Languages:
- English
- Material Type:
- Activities and Labs, Lesson Plans
- Media Format:
- Downloadable docs
- Conditions of Use:
-
Custom Permissions
All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of the copyright owner. Use solely at one's own institution with no intent for profit is excluded from the preceding copyright restriction, unless otherwise noted on the copyright notice of the individual chapter in this volume. Proper credit to this publication must be included in your laboratory outline for each use; a sample citation is given [on the copyright page of each volume]. Upon obtaining permission or with the "sole use at one's own institution" exclusion, ABLE strongly encourages individuals to use the exercises in this proceedings volume in their teaching program.
Comments