Identification of T Lymphocytes

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Author:

James M. Bader

Subject:

Science and Technology

Institution Name:

Case Western Reserve University

Collection:

Association for Biology Laboratory Education (ABLE)

Grade Level:

Post-secondary

Abstract:

In a clinical setting, it is often necessary to examine the cells of the immune system, especially in patients with immune deficiencies. One method takes advantage of the fact that lymphocytes will bind foreign red blood cells to form rosettes which can be enumerated microscopically. Specifically, the Ox49/50 antigen on the rat T lymphocyte surface has been identified as the probable binding site of the sheep rbcs. In this exercise, we will show that the number of antigen-binding lymphocytes increases after immunization.

Course Type:

Learning Module

Languages:

English

Material Type:

Activities and Labs, Lesson Plans

Media Format:

Downloadable docs

Conditions of Use:

Custom Permissions
All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of the copyright owner. Use solely at one's own institution with no intent for profit is excluded from the preceding copyright restriction, unless otherwise noted on the copyright notice of the individual chapter in this volume. Proper credit to this publication must be included in your laboratory outline for each use; a sample citation is given [on the copyright page of each volume]. Upon obtaining permission or with the "sole use at one's own institution" exclusion, ABLE strongly encourages individuals to use the exercises in this proceedings volume in their teaching program.