Identification of Bacterial Species

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Author:

Elsa Bruno, Kimberley Christopher

Subject:

Science and Technology

Institution Name:

University of Alberta

Collection:

Association for Biology Laboratory Education (ABLE)

Grade Level:

Post-secondary

Abstract:

Students are introduced to the streaking technique to isolate colonies from a mixed liquid bacteria culture and to several standard microbiological identification tests. The morphology of isolated colonies and the morphology and Gram stain reaction of the cells of each colony are examined. Each cell type is examined for the ability to use glucose as a carbon source, ability to form endospores, oxygen requirements for growth, and motility using prepared micrographs. Students compare the characteristics of the cells of the mixed bacterial culture with the same set of characteristics of six bacterial species of known identity, in an attempt at an initial identification of the bacteria of the mixed culture.

Course Type:

Learning Module

Languages:

English

Material Type:

Activities and Labs, Lesson Plans

Media Format:

Downloadable docs

Conditions of Use:

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All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of the copyright owner. Use solely at one's own institution with no intent for profit is excluded from the preceding copyright restriction, unless otherwise noted on the copyright notice of the individual chapter in this volume. Proper credit to this publication must be included in your laboratory outline for each use; a sample citation is given [on the copyright page of each volume]. Upon obtaining permission or with the "sole use at one's own institution" exclusion, ABLE strongly encourages individuals to use the exercises in this proceedings volume in their teaching program.