Honeybee Venom Detected by Enzyme Immunoassay
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- Author:
- Mary Janis
- Subject:
- Science and Technology
- Institution Name:
- University of Alaska Anchorage
- Collection:
- Association for Biology Laboratory Education (ABLE)
- Grade Level:
- Post-secondary
- Abstract:
The venom of the honeybee, Apis mellifera, contains numerous enzymes, volatiles, and other components. Allergic individuals develop anti-venom antibodies which may cause hives, anaphylactic shock, and death. In this exercise students detect concentrations of bee venom on nitrocellulose membranes following incubation of membranes with anti-venom antibodies, enzyme-conjugated secondary antibodies, and a colorimetric substrate. The intensity of the colored substrate deposited on the membrane is proportional to the original concentrations of bee venom spotted on the membrane. Students are introduced to micropipetting and to the concept of detection limits while learning about allergy and immunoassays.
- Course Type:
- Learning Module
- Languages:
- English
- Material Type:
- Activities and Labs, Lesson Plans
- Media Format:
- Downloadable docs
- Conditions of Use:
-
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