Forensic Biochemistry: DNA Testing of Plant Material
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- Author:
- Anna Wilson, Clint Chapple, Jo Cusumano, Susan J. Karcher
- Subject:
- Science and Technology
- Institution Name:
- Purdue University
- Collection:
- Association for Biology Laboratory Education (ABLE)
- Grade Level:
- Post-secondary
- Abstract:
Samples of soil were recovered from an Indiana murder victim's car and shoes. This experiment isolates DNA from plants grown from seeds that were found on the shoes. Two different ecotypes of a weed grow in different areas of Indiana. This difference can be detected using the polymerase chain reaction (PCR). DNA is extracted from the seedlings using the REDExtract-N-Amp Plant PCR Kit. Specific regions of the isolated DNA are then amplified by PCR. The PCR products are analyzed by gel electrophoresis and the results used to determine which suspect was in the same area as the victim. With data from previous exercises the students can determine who committed the murder. An alternate CTAB DNA isolation procedure is also included.
- Course Type:
- Learning Module
- Languages:
- English
- Material Type:
- Activities and Labs, Lesson Plans
- Media Format:
- Downloadable docs
- Conditions of Use:
-
Custom Permissions
All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of the copyright owner. Use solely at one's own institution with no intent for profit is excluded from the preceding copyright restriction, unless otherwise noted on the copyright notice of the individual chapter in this volume. Proper credit to this publication must be included in your laboratory outline for each use; a sample citation is given [on the copyright page of each volume]. Upon obtaining permission or with the "sole use at one's own institution" exclusion, ABLE strongly encourages individuals to use the exercises in this proceedings volume in their teaching program.
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