Examining Genetic Diversity in Disjunct Populations using Random DNA Markers

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Author:

Judith A. Guinan, Robert Sheehy

Subject:

Science and Technology

Institution Name:

Radford University

Collection:

Association for Biology Laboratory Education (ABLE)

Grade Level:

Post-secondary

Abstract:

Molecular biological techniques are used to study naturally occurring genetic variation, and have greatly facilitated the understanding of problems in many areas of biology. In this lab, we examine two populations of terrestrial isopods in the Family Armadillidiidae using Random Amplification of Polymorphic DNA (RAPD Analysis). RAPDs are robust and generally species-independent. Using this technique, we are able to detect and analyze genetic variation within and between two different populations of isopods. This lab exercise could easily be modified for use in an introductory course for majors or non-majors and would be suitable for any organism with low vagility.

Course Type:

Learning Module

Languages:

English

Material Type:

Activities and Labs, Lesson Plans

Media Format:

Downloadable docs

Conditions of Use:

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All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of the copyright owner. Use solely at one's own institution with no intent for profit is excluded from the preceding copyright restriction, unless otherwise noted on the copyright notice of the individual chapter in this volume. Proper credit to this publication must be included in your laboratory outline for each use; a sample citation is given [on the copyright page of each volume]. Upon obtaining permission or with the "sole use at one's own institution" exclusion, ABLE strongly encourages individuals to use the exercises in this proceedings volume in their teaching program.