DNA Profiling by Mutliplex PCR Amplification and Genotype Determination by Reverse Dot-Blot Hybridization to Sequence-Specific Oligonucleotide Probes

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Author:

Ralph Rascati

Subject:

Science and Technology

Institution Name:

Kennesaw State University

Collection:

Association for Biology Laboratory Education (ABLE)

Grade Level:

Post-secondary

Abstract:

Human DNA profiling has applications in paternity testing and forensics. This exercise provides students the opportunity to gain first-hand experience with procedures that are currently used to extract DNA from their own cells, quantify the DNA in the extract, perform a multiplex PCR amplification of several loci used in forensic analysis, and determine their own genotype at those loci. In addition, methods for analyzing results relative to existing population databases will be presented. The exercise is normally presented in the context of a laboratory course in Forensic DNA Analysis that presents students with a variety of techniques that have been and/or continue to be employed in forensic laboratories.

Course Type:

Learning Module

Languages:

English

Material Type:

Activities and Labs, Lesson Plans

Media Format:

Downloadable docs

Conditions of Use:

Custom Permissions
All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of the copyright owner. Use solely at one's own institution with no intent for profit is excluded from the preceding copyright restriction, unless otherwise noted on the copyright notice of the individual chapter in this volume. Proper credit to this publication must be included in your laboratory outline for each use; a sample citation is given [on the copyright page of each volume]. Upon obtaining permission or with the "sole use at one's own institution" exclusion, ABLE strongly encourages individuals to use the exercises in this proceedings volume in their teaching program.