Clearing Techniques for the Study of Vascular Plant Tissues in Whole Structures and Thick Sections

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Author:

J.M. Herr Jr.

Subject:

Science and Technology

Institution Name:

University of South Carolina

Collection:

Association for Biology Laboratory Education (ABLE)

Grade Level:

Post-secondary

Abstract:

The clearing technique presented here in 4 exercises changes the refractive properties of various organelles without altering the structural integrity of the cells. It effects a refractive uniformity among cellular organelles so that their distinctive and contrasting features can be revealed with phase contrast and Nomarski interference microscopy or through selective staining in conjunction with bright field optics.

Course Type:

Learning Module

Languages:

English

Material Type:

Activities and Labs, Lesson Plans

Media Format:

Downloadable docs

Conditions of Use:

Custom Permissions
All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of the copyright owner. Use solely at one's own institution with no intent for profit is excluded from the preceding copyright restriction, unless otherwise noted on the copyright notice of the individual chapter in this volume. Proper credit to this publication must be included in your laboratory outline for each use; a sample citation is given [on the copyright page of each volume]. Upon obtaining permission or with the "sole use at one's own institution" exclusion, ABLE strongly encourages individuals to use the exercises in this proceedings volume in their teaching program.