Bacterial Gene Transfer Chapter 3

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Author:

John Mordacq, Roberta Ellington

Subject:

Science and Technology

Institution Name:

Northwestern University

Collection:

Association for Biology Laboratory Education (ABLE)

Grade Level:

Post-secondary

Abstract:

This laboratory investigates one form of genetic recombination in bacteria. This process, called conjugation, occurs when one bacteria transfers DNA to another bacteria. Two different strains of Escherichia coli are used: an Hfr strain with the F factor integrated into the bacterial chromosome acting as the donor, and an F-strain lacking the fertility factor acting as a recipient. The F-strain is auxotrophic for certain genetic markers and the ordered transfer of markers from the Hfr strain to the F-strain is used to map gene locations on the bacterial chromosome.

Course Type:

Learning Module

Languages:

English

Material Type:

Activities and Labs, Lesson Plans

Media Format:

Downloadable docs

Conditions of Use:

Custom Permissions
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