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Immunocytochemical Analysis of Sperm Cytoskeleton
(Complete Item Description)
- Abstract:
Monoclonal antitubulin antibodies produced in mice will be used to label bull sperm microtubules and the location of these antibodies will be determined by use of a peroxidase conjugated secondary antibody and the DAB staining reaction. Protocols are presented for use in a second-year student laboratory class given in a multi-section (repeated) format. These protocols include those for preparation of poly-L-lysine coated slides and of bull sperm, as well as those for immunocytochemistry, using the DAB reaction.
- Subject:
- Science and Technology
- Grade Level:
- Post-secondary
- Collection:
- Association for Biology Laboratory Education (ABLE)
