Examines the people and construction of Bent's Fort, and the Santa Fe Trail. Built originally in 1833, this adobe fort became a center of trade with Indians and trappers. For much of its 16-year history, it was the only major permanent white settlement on the Santa Fe Trail. It provided explorers, adventurers, and the U.S. Army a place to get supplies, wagon repairs, livestock, good food, water, company, rest and protection in this vast Great American Desert.
Students perform Western blots with polyacrylamide gel electrophoresis of detergent extracts of SV40-transformed cell lines grown in monolayer culture. The gels are blotted onto nitrocellulose and treated with a monoclonal antibody against SV40 T antigen or against the tumor suppressor p53. Blots are then treated with a secondary antibody (anti-mouse IgG conjugated to horseradish peroxidase), and exposed to revealing chemicals and x-ray film. I give one application: induction of p53 by ultraviolet light.
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