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Teaching Western Blots with T antigen and p53
(Complete Item Description)
- Abstract:
Students perform Western blots with polyacrylamide gel electrophoresis of detergent extracts of SV40-transformed cell lines grown in monolayer culture. The gels are blotted onto nitrocellulose and treated with a monoclonal antibody against SV40 T antigen or against the tumor suppressor p53. Blots are then treated with a secondary antibody (anti-mouse IgG conjugated to horseradish peroxidase), and exposed to revealing chemicals and x-ray film. I give one application: induction of p53 by ultraviolet light.
- Subject:
- Science and Technology
- Grade Level:
- Post-secondary
- Collection:
- Association for Biology Laboratory Education (ABLE)
