Keywords: Molecular Biology (160)

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Detection of Genetically Modified Foods

Detection of Genetically Modified Foods

Genetically modified foods are often in the news and widely grown in ... (more)

Genetically modified foods are often in the news and widely grown in the United States. Three US government agencies (USDA, FDA, and EPA) work to regulate the introduction and production of genetically modified foods. These crops can provide agricultural, ecological and nutritional benefits, but there are also potential risks to the environment and consumers. As consumers and public interest groups around the world have become aware of these risks, there has been a call for more explicit product labeling and reliable methods for the detection of genetic modification in the foods we eat. This lab activity explores these issues by taking students through a three-part process to detect the presence of genetic modification in corn (maize) or soy food products. This lab uses one of the two methods for detection of genetic modification currently approved by the European Union. (less)

Subject:
Science and Technology
Material Type:
Activities and Labs
Lesson Plans
Collection:
Association for Biology Laboratory Education (ABLE)
Provider:
Madison Area Technical College
Author:
Diana Brandner
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Do You Really Know What You're Eating?: A Case Study on Genetically Modified Foods

Do You Really Know What You're Eating?: A Case Study on Genetically Modified Foods

Starting from a fictional "news" report about an apparent allergic reaction to ... (more)

Starting from a fictional "news" report about an apparent allergic reaction to a taco tainted by genetically modified corn, students consider some of the techniques and procedures used in modern molecular genetics and microbiology as well as some of the issues associated with genetically modified organisms (GMOs). Originally designed for role-play and PowerPoint assignments, suggestions for a shortened version are also provided. Suitable for a general microbiology course, the case could also be used in an introductory molecular biology course with appropriate modifications. Various levels of coverage of the topic of recombinant DNA are possible. (less)

Subject:
Science and Technology
Social Sciences
Material Type:
Activities and Labs
Case Study
Lesson Plans
Collection:
Case Study Teaching in Science
Provider:
National Center for Case Study Teaching in Science
Author:
Mary Celeste Reese
Wayne Shew
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The Double Life of RNA, Lecture 1: Catalysis: Chemical and Biochemical

The Double Life of RNA, Lecture 1: Catalysis: Chemical and Biochemical

This lecture covers the differences between chemical and biochemical reactions, an overview ... (more)

This lecture covers the differences between chemical and biochemical reactions, an overview of RNA, and the principles of catalysis. (less)

Subject:
Science and Technology
Material Type:
Video Lectures
Collection:
Howard Hughes Medical Institute
Provider:
Howard Hughes Medical Institute
Author:
Thomas R. Cech, Ph.D.
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The Double Life of RNA, Lecture 3: How to Accelerate a Reaction a Hundred Billion Times Using Only RNA

The Double Life of RNA, Lecture 3: How to Accelerate a Reaction a Hundred Billion Times Using Only RNA

This lecture provides information about how folded RNA molecules provide both the ... (more)

This lecture provides information about how folded RNA molecules provide both the high specificity and tremendous rate acceleration that is characteristic of catalysis, as well as the structure of RNA that promotes these reactions. (less)

Subject:
Science and Technology
Material Type:
Video Lectures
Collection:
Howard Hughes Medical Institute
Provider:
Howard Hughes Medical Institute
Author:
Thomas R. Cech, Ph.D.
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Examining Genetic Diversity in Disjunct Populations using Random DNA Markers

Examining Genetic Diversity in Disjunct Populations using Random DNA Markers

Molecular biological techniques are used to study naturally occurring genetic variation, and ... (more)

Molecular biological techniques are used to study naturally occurring genetic variation, and have greatly facilitated the understanding of problems in many areas of biology. In this lab, we examine two populations of terrestrial isopods in the Family Armadillidiidae using Random Amplification of Polymorphic DNA (RAPD Analysis). RAPDs are robust and generally species-independent. Using this technique, we are able to detect and analyze genetic variation within and between two different populations of isopods. This lab exercise could easily be modified for use in an introductory course for majors or non-majors and would be suitable for any organism with low vagility. (less)

Subject:
Science and Technology
Material Type:
Activities and Labs
Lesson Plans
Collection:
Association for Biology Laboratory Education (ABLE)
Provider:
Radford University
Author:
Judith A. Guinan
Robert Sheehy
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Experimental Molecular Neurobiology, Fall 2006

Experimental Molecular Neurobiology, Fall 2006

Designed for students without previous experience in techniques of cellular and molecular ... (more)

Designed for students without previous experience in techniques of cellular and molecular biology, this class teaches basic experimental techniques in cellular and molecular neurobiology. Experimental approaches covered include tissue culture of neuronal cell lines, dissection and culture of brain cells, DNA manipulation, synaptic protein analysis, immunocytochemistry, and fluorescent microscopy. (less)

Subject:
Science and Technology
Material Type:
Activities and Labs
Full Course
Homework and Assignments
Syllabi
Collection:
MIT OpenCourseWare
Provider:
M.I.T.
Author:
Hayashi, Yasunori
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Experiments with the Structure and Function of DNA

Experiments with the Structure and Function of DNA

This exercise will give students an interactive opportunity to develop their understanding ... (more)

This exercise will give students an interactive opportunity to develop their understanding of DNA structure, how it is packaged into chromosomes, and the mechanisms of transcription and translation. (less)

Subject:
Science and Technology
Material Type:
Activities and Labs
Lesson Plans
Collection:
Association for Biology Laboratory Education (ABLE)
Provider:
Clemson University
Author:
Doris R. Helms
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Expression Vectors Used in Project-Oriented Teaching Laboratories Chapter 1

Expression Vectors Used in Project-Oriented Teaching Laboratories Chapter 1

This resource is designed to provide a framework for developing laboratory courses ... (more)

This resource is designed to provide a framework for developing laboratory courses in cellular and molecular biology, and to provide scientific and technological standards for use in research and teaching laboratories. (less)

Subject:
Science and Technology
Material Type:
Activities and Labs
Lesson Plans
Collection:
Association for Biology Laboratory Education (ABLE)
Provider:
Purdue University
Author:
Chris J. Staiger
David J. Asai
Susan J. Karcher
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Expression Vectors Used in Project-Oriented Teaching Laboratories Chapter 2

Expression Vectors Used in Project-Oriented Teaching Laboratories Chapter 2

This chapter describes a multi-week project taught in an upper level cell ... (more)

This chapter describes a multi-week project taught in an upper level cell and molecular biology laboratory. Isolation of sufficient quantities of rare proteins from biological tissues can be difficult. These native proteins are often required for raising antibodies, and for studying enzymatic or regulatory function. Expression vectors are used to obtain large quantities of proteins. The cDNA encoding a protein is cloned into an expression vector. The fusion protein is over-expressed in E. coli, and large quantities of the recombinant protein are obtained by affinity chromatography. SDS-polyacrylamide gel electrophoresis is used to determine the size of the proteins isolated. (less)

Subject:
Science and Technology
Material Type:
Activities and Labs
Lesson Plans
Collection:
Association for Biology Laboratory Education (ABLE)
Provider:
Harvey Mudd College|Purdue University
Author:
Chris J. Staiger
David J. Asai
Susan J. Karcher
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Extracting DNA

Extracting DNA

This lesson for students in grades 9-12 introduces DNA, genes, chromosomes, the ... (more)

This lesson for students in grades 9-12 introduces DNA, genes, chromosomes, the chemicals that make up DNA. After the basic information, students will do an experiment in which they will separate out DNA from peas. Knowing that DNA can be separated will give them a base of understanding for future lessons in biology, evolution, biotechnology, and health technology. (less)

Subject:
Science and Technology
Material Type:
Lesson Plans
Teaching and Learning Strategies
Collection:
Science Netlinks
Provider:
AAAS
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A Family in Need: In-Class Case Study on Cancer Genetics

A Family in Need: In-Class Case Study on Cancer Genetics

“A Family in Need” was designed as an in-class problem-based learning activity ... (more)

“A Family in Need” was designed as an in-class problem-based learning activity for students to learn about several innovative medical applications of molecular biology. Students assume the role of a second-year medical student assigned to work with a pediatric oncologist who has just biopsied a tumor-like growth in the adrenal gland of her 17-year-old patient, Lee F. After taking Lee’s family history and performing a pedigree analysis, students review clinical and genetic characteristics of several syndromes associated with adrenal cancer. Students then explore various diagnostic and biomedical research techniques such as PCR, DNA sequencing, and pre-implantation genetic diagnosis. The case concludes with a consideration of how to treat Lee’s condition with the help of gene cloning and the potential of gene therapy. Although originally written for an upper-level college genetics course, the case could also be adapted for an introductory molecular/cellular biology course, a non-majors biology course, or a professional school medical genetics course. (less)

Subject:
Science and Technology
Material Type:
Case Study
Lecture Notes
Unit of Study
Collection:
Case Study Teaching in Science
Provider:
National Center for Case Study Teaching in Science
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Fields, Forces and Flows in Biological Systems, Spring 2007

Fields, Forces and Flows in Biological Systems, Spring 2007

This course introduces the basic driving forces for electric current, fluid flow, ... (more)

This course introduces the basic driving forces for electric current, fluid flow, and mass transport, plus their application to a variety of biological systems. Basic mathematical and engineering tools will be introduced, in the context of biology and physiology. Various electrokinetic phenomena are also considered as an example of coupled nature of chemical-electro-mechanical driving forces. Applications include transport in biological tissues and across membranes, manipulation of cells and biomolecules, and microfluidics. (less)

Subject:
Science and Technology
Material Type:
Full Course
Homework and Assignments
Lecture Notes
Syllabi
Collection:
MIT OpenCourseWare
Provider:
M.I.T.
Author:
Han, Jongyoon (Jay)
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Forensic Biochemistry: DNA Testing of Plant Material

Forensic Biochemistry: DNA Testing of Plant Material

Samples of soil were recovered from an Indiana murder victim's car and ... (more)

Samples of soil were recovered from an Indiana murder victim's car and shoes. This experiment isolates DNA from plants grown from seeds that were found on the shoes. Two different ecotypes of a weed grow in different areas of Indiana. This difference can be detected using the polymerase chain reaction (PCR). DNA is extracted from the seedlings using the REDExtract-N-Amp Plant PCR Kit. Specific regions of the isolated DNA are then amplified by PCR. The PCR products are analyzed by gel electrophoresis and the results used to determine which suspect was in the same area as the victim. With data from previous exercises the students can determine who committed the murder. An alternate CTAB DNA isolation procedure is also included. (less)

Subject:
Science and Technology
Material Type:
Activities and Labs
Lesson Plans
Collection:
Association for Biology Laboratory Education (ABLE)
Provider:
Purdue University
Author:
Anna Wilson
Clint Chapple
Jo Cusumano
Susan J. Karcher
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Freshman Seminar: Structural Basis of Genetic Material: Nucleic Acids, Fall 2005

Freshman Seminar: Structural Basis of Genetic Material: Nucleic Acids, Fall 2005

Since the discovery of the structure of the DNA double helix in ... (more)

Since the discovery of the structure of the DNA double helix in 1953 by Watson and Crick, the information on detailed molecular structures of DNA and RNA, namely, the foundation of genetic material, has expanded rapidly. This discovery is the beginning of the "Big Bang" of molecular biology and biotechnology. In this seminar, students discuss, from a historical perspective and current developments, the importance of pursuing the detailed structural basis of genetic materials. (less)

Subject:
Science and Technology
Material Type:
Full Course
Homework and Assignments
Syllabi
Collection:
MIT OpenCourseWare
Provider:
M.I.T.
Author:
Zhang, Shuguang
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Gene Expression During Development: Experimental Design Problem

Gene Expression During Development: Experimental Design Problem

This problem challenges students to design experiments using techniques measuring gene expression ... (more)

This problem challenges students to design experiments using techniques measuring gene expression (reverse transcriptase PCR, microarrays, in situ hybridization). (less)

Subject:
Science and Technology
Material Type:
Activities and Labs
Collection:
Starting Point (SERC)
Provider:
SERC at Carlton College
Author:
Debby Walser-Kuntz
Sarah Deel
Susan Singer
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General Biology I

General Biology I

An integrated course stressing the principles of biology. Life processes are examined ... (more)

An integrated course stressing the principles of biology. Life processes are examined primarily at the molecular and cellular levels. Intended for students majoring in biology or for non-majors who wish to take advanced biology courses. (less)

Subject:
Science and Technology
Material Type:
Activities and Labs
Full Course
Lecture Notes
Syllabi
Collection:
UMass Boston OpenCourseWare
Provider:
UMass Boston
Author:
Ph.D.
Professor Brian White
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Genetic Control of Cell Chemistry Using Serratia marcescens

Genetic Control of Cell Chemistry Using Serratia marcescens

This exercise allows students to investigate the genetic control of a biosynthetic ... (more)

This exercise allows students to investigate the genetic control of a biosynthetic pathway: the production of the red pigment prodigiosin by the bacterium Serratia marcescens. The students perform feeding trials with mutant strains to examine the genetic control of this biosynthetic pathway. Auxotrophic strains of the bacteria which are blocked at different places in the pathway exhibit different colors, the wild-type is a deep red color. Pairwise feeding trials enable the students to determine which strains are blocked at which point in the pathway. Students will then use an explanatory system to determine the pathway's shape and learn about its genetic control. (less)

Subject:
Science and Technology
Material Type:
Activities and Labs
Lesson Plans
Collection:
Association for Biology Laboratory Education (ABLE)
Provider:
University of Nevada
Author:
Elise Schmidt
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