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Mapping Genes in C. elegans

Abstract:: The advantages of using Caenorhabditis elegans in introductory genetics courses will be demonstrated in this exercise in which an "unknown" visible mutation will be assigned to a linkage group and mapped relative to known markers. The nematode can be cultured easily, has a short generation time, reproduces either as self-fertilizing hermaphrodites or in outcrosses yields large numbers of progeny, and has a simple genome.

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A Study of Gene Linkage and Mapping Using Tetrad Analysis in the Fungus Sordaria fimicola

Abstract:: This laboratory focuses on some aspects of spore color determination in the fungus Sordaria fimicola. Tetrad analysis of asci from a cross between strains mutant for two spore color genes is used to determine if the genes are linked and to map the location of each gene relative to the centromere. This laboratory is logistically simple and inexpensive, the data are relatively easy to collect, and the analysis facilitates a good understanding of meiosis and Mendel's principles of inheritance. Alternate approaches to the use of this laboratory are discussed.