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Directed Evolution: Engineering Biocatalysts, Spring 2008

Directed Evolution: Engineering Biocatalysts, Spring 2008

Enzymes, nature's catalysts, are remarkable biomolecules capable of extraordinary specificity and selectivity. ... (more)

Enzymes, nature's catalysts, are remarkable biomolecules capable of extraordinary specificity and selectivity. Directed evolution has been used to produce enzymes with many unique properties, including altered substrate specificity, thermal stability, organic solvent resistance, and enantioselectivity--selectivity of one stereoisomer over another. The technique of directed evolution comprises two essential steps: mutagenesis of the gene encoding the enzyme to produce a library of variants, and selection of a particular variant based on its desirable catalytic properties. In this course we will examine what kinds of enzymes are worth evolving and the strategies used for library generation and enzyme selection. We will focus on those enzymes that are used in the synthesis of drugs and in biotechnological applications. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literature to discuss and learn about current biological research in a highly interactive setting. Many instructors of the Advanced Undergraduate Seminars are postdoctoral scientists with a strong interest in teaching. (less)

Subject:
Science and Technology
Material Type:
Full Course
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Lecture Notes
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Collection:
MIT OpenCourseWare
Provider:
M.I.T.
Author:
Love, Kerry
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2002 llaF ,gnivloS melborP gnireenignE dna sretupmoC ot noitcudortnI

.desu si egaugnal gnimmargorp avaJ ehT .gninnalp dna ,tnemeganam ,ecneics ,gnireenigne ni ... (more)

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Subject:
Science and Technology
Material Type:
Assessments
Full Course
Homework and Assignments
Lecture Notes
Syllabi
Collection:
MIT OpenCourseWare
Provider:
M.I.T.
Author:
George Kocur
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