This resource is a video abstract of a research paper created by Research Square on behalf of its authors. It provides a synopsis that's easy to understand, and can be used to introduce the topics it covers to students, researchers, and the general public. The video's transcript is also provided in full, with a portion provided below for preview:
"When scientists want to understand microbial populations, they turn to metagenomics. The standard technique, shotgun metagenomics, produces nearly complete genomes, enabling researchers to predict traits within a species. Unfortunately, this method applies to broad populations, making it difficult to precisely link metabolic traits to individual species. Sequencing single cells addresses this shortcoming, but the information provided is often incomplete. Now, researchers have developed a way to target smaller populations for metagenomic sequencing. Before sequencing, the researchers added a critical sorting step using hybridization chain reaction fluorescence in-situ hybridization (HCR-FISH). Isolating a population of interest before the sorted cells were used for shotgun sequencing. The result was a more complete genome of a targeted species with low diversity. The technique provided the team enough precision to analyze metabolic features of bacteria sparsely found feeding on decaying diatom algae..."
The rest of the transcript, along with a link to the research itself, is available on the resource itself.
- Subject:
- Biology
- Life Science
- Material Type:
- Diagram/Illustration
- Reading
- Provider:
- Research Square
- Provider Set:
- Video Bytes
- Date Added:
- 04/29/2020