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(Complete Item Description)
- Abstract:
Subject uses molecular genetics to examine how Gram-positive and Gram-negative bacteria can be used in novel and relevant bioconversion processes, for example, to synthesize precursors to the drug Crixivan, a potent inhibitor of HIV replication, or to synthesize metabolites as food supplements. Students engage in independent research projects to address questions relating to these processess. Techniques used include plasmid manipulation, genetic complementation, mutagenesis, PCR, and DNA sequencing, enzyme assays, and gene expression studies. Instruction and practice in written and oral communication provided. Also referred to as the Microbial Genetics Project Lab, this is a hands-on research course designed to introduce the student to the strategies and challenges associated with microbiology research. Students take on independent and original research projects that are designed to be instructive with the goal of advancing a specific field of research in microbiology.
- Subject:
- Science and Technology
- Grade Level:
- Post-secondary
- Collection:
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MIT OpenCourseWare
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(Complete Item Description)
- Abstract:
" In this class, students engage in independent research projects to probe various aspects of the physiology of the bacterium Pseudomonas aeruginosa PA14, an opportunistic pathogen isolated from the lungs of cystic fibrosis patients. Students use molecular genetics to examine survival in stationary phase, antibiotic resistance, phase variation, toxin production, and secondary metabolite production. Projects aim to discover the molecular basis for these processes using both classical and cutting-edge techniques. These include plasmid manipulation, genetic complementation, mutagenesis, PCR, DNA sequencing, enzyme assays, and gene expression studies. Instruction and practice in written and oral communication are also emphasized. WARNING NOTICE The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such safety procedures and measures. MIT shall have no responsibility, liability, or risk for the content or implementation of any of the material presented. Legal Notice "
- Subject:
- Science and Technology
- Grade Level:
- Post-secondary
- Collection:
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MIT OpenCourseWare
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