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Read the Fine Print
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(Complete Item Description)
- Abstract:
This laboratory investigates one form of genetic recombination in bacteria. This process, called conjugation, occurs when one bacteria transfers DNA to another bacteria. Two different strains of Escherichia coli are used: an Hfr strain with the F factor integrated into the bacterial chromosome acting as the donor, and an F-strain lacking the fertility factor acting as a recipient. The F-strain is auxotrophic for certain genetic markers and the ordered transfer of markers from the Hfr strain to the F-strain is used to map gene locations on the bacterial chromosome.
- Subject:
- Science and Technology
- Grade Level:
- Post-secondary
- Collection:
-
Association for Biology Laboratory Education (ABLE)
Read the Fine Print
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Read the Fine Print
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(Complete Item Description)
- Abstract:
This chapter describes a multi-week project taught in an upper level cell and molecular biology laboratory. Isolation of sufficient quantities of rare proteins from biological tissues can be difficult. These native proteins are often required for raising antibodies, and for studying enzymatic or regulatory function. Expression vectors are used to obtain large quantities of proteins. The cDNA encoding a protein is cloned into an expression vector. The fusion protein is over-expressed in E. coli, and large quantities of the recombinant protein are obtained by affinity chromatography. SDS-polyacrylamide gel electrophoresis is used to determine the size of the proteins isolated.
- Subject:
- Science and Technology
- Grade Level:
- Post-secondary
- Collection:
-
Association for Biology Laboratory Education (ABLE)
Remix and Share
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(Complete Item Description)
- Abstract:
The need to identify sustainable forms of energy as an alternative to our dependence on depleting worldwide oil reserves is one of the grand challenges of our time. The energy from the sun converted into plant biomass is the most promising renewable resource available to humanity. This seminar will examine each of the critical steps along the pathway towards the conversion of plant biomass into ethanol. This course is one of many Advanced Undergraduate Seminars offered by the Biology Department at MIT. These seminars are tailored for students with an interest in using primary research literature to discuss and learn about current biological research in a highly interactive setting. Many instructors of the Advanced Undergraduate Seminars are postdoctoral scientists with a strong interest in teaching.
- Subject:
- Science and Technology
- Grade Level:
- Post-secondary
- SubTopics:
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Energy
- Collection:
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MIT OpenCourseWare
Read the Fine Print
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(Complete Item Description)
- Abstract:
Build a gene network! The lac operon is a set of genes which are responsible for the metabolism of lactose in some bacterial cells. Explore the effects of mutations within the lac operon by adding or removing genes from the DNA.
- Subject:
- Mathematics and Statistics, Science and Technology
- Grade Level:
- Secondary, Post-secondary
- Collection:
-
PhET Interactive Simulations
Read the Fine Print
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(Complete Item Description)
- Abstract:
Recovery of Escherichia coli from a rectal swab begins a series of procedures combining mastery of technique with productive experiments. After isolating his/her own E. coli strain, each student recovers a unique coliphage from raw sewage and determines its host range, thereby revealing the genetic individuality of each bacterial and viral isolate. Colonies of virus-resistant mutants can often be recovered in regions where sensitive bacteria have been destroyed. After antibiotic sensitivity testing of E. coli isolates, a variety of antibiotic resistant mutants can be recovered, thus yielding genetically marked strains for future bacterial genetics experiments.
- Subject:
- Science and Technology
- Grade Level:
- Post-secondary
- Collection:
-
Association for Biology Laboratory Education (ABLE)
Read the Fine Print
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(Complete Item Description)
- Abstract:
Two strains of Escherichia coli are grown in broth and plated out at intervals. A population growth curve is plotted for each strain. The two strains are then grown together for several days in different types of broth. Students from each day's laboratory plate out the mixed culture on selective media. Data are pooled. Population curves are plotted for the two strains in competition. With different conditions, it is possible to observe competitive displacement of one strain by another.
- Subject:
- Science and Technology
- Grade Level:
- Post-secondary
- SubTopics:
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Green
- Collection:
-
Association for Biology Laboratory Education (ABLE)
Read the Fine Print
-
(Complete Item Description)
- Abstract:
This textbook has evolved from online and live-in-person lectures presented in my bacteriology courses at the University of Wisconsin-Madison. Its contents are suitable for reading or presentation in courses or course modules concerning general microbiology and medical bacteriology at the college and advanced high school levels of education. For teachers, instructional materials are available that accompany many chapters and topics. These include lecture outlines, notes, powerpoint presentations, and examination questions that compose a study guide
- Subject:
- Science and Technology
- Grade Level:
- Post-secondary
- Collection:
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University of Wisconsin-Madison
