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Murder and Mayhem in Non-Majors Biology

Abstract:: Who killed James Watson in the biology lab, with the biology textbook? In this non-majors laboratory exercise, students use scientific inquiry skills to solve a murder mystery. Many are suspects, but only one committed the crime. Each student plays a role and tries to uncover motive and opportunity of the other suspects. Hypotheses are tested with physical evidence: fingerprints, blood type, and paper strip DNA analysis.

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Use PCR and a Single Hair to Produce a "DNA Fingerprint"

Abstract:: As a former student once commented, "PCR is the sliced bread of molecular biology". Polymerase Chain Reaction (PCR) is a process that allows an investigator to amplify a portion of a single piece of DNA into over one billion copies. We have used PCR with our Introductory Biology students to generate a simple "DNA fingerprint". This exercise will outline all the steps required to generate a DNA fingerprint for each student during two lab meetings. This article covers the background information concerning the relevant techniques and the specific details required for students to isolate their own DNA from a single hair follicle, prepare their DNA for PCR, load an agarose gel, and analyze their DNA samples.