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Read the Fine Print
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(Complete Item Description)
- Abstract:
Cells contain many specialized structures known as organelles which perform a variety of specific functions. To obtain precise information about the structure and functions of subcellular organelles, it is imperative to isolate them free from contaminating organelles. Marker enzymes are known to be localized exclusively in specific organelles and are used to monitor the separation of these organelles. In this exercise the homogenate of Dictyostelium discoideum will be fractionated by differential centrifugation to separate mitochondria, lysosomes, and contractile vacuoles. The fractions will be assayed for their marker enzymes: acid phosphatase for lysosomes, succinate dehydrogenase for mitochondria, and alkaline phosphodiesterase for the contractile vacuoles. This exercise presents two fundamental concepts to the students: organelle isolation and enzyme assays.
- Subject:
- Science and Technology
- Grade Level:
- Post-secondary
- Collection:
-
Association for Biology Laboratory Education (ABLE)
Read the Fine Print
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(Complete Item Description)
- Abstract:
Microbial eukaryotes in general, and cellular slime molds in particular, are well-suited for laboratory instruction in a variety of areas, including cellular biology, developmental biology, biochemistry, and population biology, and for a range of educational levels, such as introductory courses, upper-division courses, and independent student research projects. This chapter details methods for the culturing and care of cellular slime molds, as well as instructions for several experiments and explorations including an examination of the life cycle, how cells choose a developmental pathway, food preferences and interspecific interactions, chemotaxis, phototaxis, pattern formation, and cell recognition.
- Subject:
- Science and Technology
- Grade Level:
- Post-secondary
- SubTopics:
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Green
- Collection:
-
Association for Biology Laboratory Education (ABLE)
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