Keywords: Copper-Tolerant Mutants (1)

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When Do Adaptive Mutants Arise in Yeast?

When Do Adaptive Mutants Arise in Yeast?

This exercise examines when adaptive mutations arise in a population. Students prepare ... (more)

This exercise examines when adaptive mutations arise in a population. Students prepare cultures of baker's yeast on normal agar media and then transfer the exact spatial pattern of these colonies to agar plates containing copper using the replica-plating technique. They compare the pattern of mutant colonies on the two replicate plates to test when copper-tolerant mutants arose. If the colony positions match then the mutants pre-existed in the original population (spontaneous mutation). If colonies do not match then this supports a hypothesis that mutants were induced by exposure to the copper. Students learn skills such as preparing agar plates, the sterile technique, replica-plating, and the use of pipets and dilutions. (less)

Subject:
Science and Technology
Material Type:
Activities and Labs
Lesson Plans
Collection:
Association for Biology Laboratory Education (ABLE)
Provider:
University of Toronto
Author:
Corey A. Goldman
Michael Dennison
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2002 llaF ,gnivloS melborP gnireenignE dna sretupmoC ot noitcudortnI

.desu si egaugnal gnimmargorp avaJ ehT .gninnalp dna ,tnemeganam ,ecneics ,gnireenigne ni ... (more)

.desu si egaugnal gnimmargorp avaJ ehT .gninnalp dna ,tnemeganam ,ecneics ,gnireenigne ni smelborp gnivlos rof seuqinhcet gnipoleved no si sisahpmE .scipot decnavda detceles dna scihparg retupmoc ,gnihcraes dna gnitros ,serutcurts atad ,sdohtem laciremun ,secafretni resu lacihparg ,stpecnoc gnimmargorp revoc smelborp gnimmargorp ylkeeW .esruoc eht fo sucof eht si tnempoleved dna ngised erawtfos detneiro-tcejbO .snoitacilppa cifitneics dna gnireenigne rof sdohtem lanoitatupmoc dna tnempoleved erawtfos latnemadnuf stneserp esruoc sihT (less)

Subject:
Science and Technology
Material Type:
Assessments
Full Course
Homework and Assignments
Lecture Notes
Syllabi
Collection:
MIT OpenCourseWare
Provider:
M.I.T.
Author:
George Kocur
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