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Read the Fine Print
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(Complete Item Description)
- Abstract:
This laboratory investigates one form of genetic recombination in bacteria. This process, called conjugation, occurs when one bacteria transfers DNA to another bacteria. Two different strains of Escherichia coli are used: an Hfr strain with the F factor integrated into the bacterial chromosome acting as the donor, and an F-strain lacking the fertility factor acting as a recipient. The F-strain is auxotrophic for certain genetic markers and the ordered transfer of markers from the Hfr strain to the F-strain is used to map gene locations on the bacterial chromosome.
- Subject:
- Science and Technology
- Grade Level:
- Post-secondary
- Collection:
-
Association for Biology Laboratory Education (ABLE)
Remix and Share
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(Complete Item Description)
- Abstract:
At the end of this lesson you can describe the way to your home in a e-mail. You practice conjugation of verbs.
- Subject:
- Humanities
- Grade Level:
- Secondary
- Collection:
-
KlasCement
Remix and Share
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(Complete Item Description)
- Abstract:
At the completion of this lesson you will be able to ask for the price of a drink and order a drink. You will practice verb conjugation in the various tenses
- Subject:
- Humanities
- Grade Level:
- Post-secondary
- Collection:
-
KlasCement
Read the Fine Print
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(Complete Item Description)
- Abstract:
Creation and characterization of mutants is the basis for any genetic analysis. This exercise demonstrates a simple, safe procedure for transposon mutagenesis of Rhodobacter sphaeroides, a purple non-sulfur photosynthetic bacteria. Students perform the mutagenesis by mating a transposon-carrying plasmid from Escherichia coli to R. sphaeroides and then selecting for the drug resistance carried on the transposon. Only R. sphaeroides carrying the transposon in the chromosome survive the selection. Transposon carrying mutants are then scored for various phenotypes. The metabolic diversity of R. sphaeroides allows the isolation of nutritional, photopigment, and photosynthetic mutants. Further analysis of mutants is possible.
- Subject:
- Science and Technology
- Grade Level:
- Post-secondary
- Collection:
-
Association for Biology Laboratory Education (ABLE)
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