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Read the Fine Print
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(Complete Item Description)
- Abstract:
To illustrate cell fractionation, nuclei are isolated from the ciliated protozoan, Tetrahymena thermophila. A table top clinical centrifuge is used for the fractionation steps and the procedure is monitored microscopically using a differential stain. To determine the efficiency of the procedure, cell and nuclear counts are determined with a hemacytometer. To quantify DNA, the Diphenylamine Reaction is carried out and the amount of DNA per nucleus is calculated.
- Subject:
- Science and Technology
- Grade Level:
- Post-secondary
- Collection:
-
Association for Biology Laboratory Education (ABLE)
Read the Fine Print
-
Read the Fine Print
-
(Complete Item Description)
- Abstract:
Fractions of plant cells prepared by homogenization and differential centrifugation exhibit enzymatic activity. In fractions containing chloroplasts, light causes evolution of oxygen and generation of high-energy electrons that are passed along the electron transport chain of the thylakoid membrane. The results of an assay for the reduction of DCPIP, an artificial electron acceptor, in these fractions can illustrate the photosynthetic activity that takes place within the living cell, and can indicate how successful the fractionation procedure has been in separating organelles.
- Subject:
- Science and Technology
- Grade Level:
- Post-secondary
- Collection:
-
Association for Biology Laboratory Education (ABLE)
Read the Fine Print
-
(Complete Item Description)
- Abstract:
By correlating the protein concentration of various fractions from E. coli with the appearance of samples on a gel, this exercise introduces beginning students to the quantitative "bookkeeping" involved in protein purification. A periplasmic fraction is isolated by osmotic shock, and affinity chromatography with amylose resin is used to purify maltose-binding protein. Visualization of fractions by SDS-polyacrylamide gel electrophoresis allows students to evaluate the qualitative success of the purification and to estimate the subunit molecular weight of the purified protein.
- Subject:
- Science and Technology
- Grade Level:
- Post-secondary
- Collection:
-
Association for Biology Laboratory Education (ABLE)
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