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Read the Fine Print
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(Complete Item Description)
- Abstract:
This laboratory exercise is designed to illustrate the semi-conservative mode of DNA duplication through the expression of sister chromatid differentiation (SCD), the single-stranded nature of the eukaryotic chromosome organization, and the mutagenic effects of certain chemical or physical agents through induced sister chromatid exchange (SCE). Through individual work and/or a group project, students will learn the basic cell culturing and cytogenetic techniques for chromosome studies, especially the Fluorescence Plus Giemsa (FPG) technique for detecting SCD and SCE. Comprehensive background and technical information and notes for the instructor are included.
- Subject:
- Science and Technology
- Grade Level:
- Post-secondary
- Collection:
-
Association for Biology Laboratory Education (ABLE)
Read the Fine Print
-
(Complete Item Description)
- Abstract:
Students perform Western blots with polyacrylamide gel electrophoresis of detergent extracts of SV40-transformed cell lines grown in monolayer culture. The gels are blotted onto nitrocellulose and treated with a monoclonal antibody against SV40 T antigen or against the tumor suppressor p53. Blots are then treated with a secondary antibody (anti-mouse IgG conjugated to horseradish peroxidase), and exposed to revealing chemicals and x-ray film. I give one application: induction of p53 by ultraviolet light.
- Subject:
- Science and Technology
- Grade Level:
- Post-secondary
- Collection:
-
Association for Biology Laboratory Education (ABLE)
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