The advantages of using Caenorhabditis elegans in introductory genetics courses will be demonstrated in this exercise in which an "unknown" visible mutation will be assigned to a linkage group and mapped relative to known markers. The nematode can be cultured easily, has a short generation time, reproduces either as self-fertilizing hermaphrodites or in outcrosses yields large numbers of progeny, and has a simple genome.
C. elegans is easy to use, transparent, reproduces quickly, and has a cloned genome. All of these factors make it an ideal system for use in introductory, genetics or developmental biology labs. This lab familiarizes students with the life cycle, development, terminology, and common mutant phenotypes of C. elegans. It also introduces the use of GFP transgenic worms to visualize specific cell types in the worm. A detailed sample experiment is included that takes students through using genetic crosses and GFP transgenic strains to figure out the cause of a mutant phenotype.
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