Spot-Overlay Ames Test of Potential Mutagens

Abstract:

This protocol represents a cost-effective modification of the Ames Test that allows students to investigate the mutagenic potential of various common substances. Potential mutagens are tested using well-characterized auxotrophic strains of Salmonella typhimurium. By analyzing the results, students determine if any of their compounds may be mutagenic. Follow-up experiments are designed to determine the dose response of these potential mutagens. Using this protocol, we have achieved reproducible results with several known mutagens, including sodium azide and ultraviolet irradiation. This approach enables faculty to control costs and results to improve student understanding of mutagenesis, biochemical pathways, experimental design, and data analysis.

Subject:

Science and Technology

Grade Level:

Secondary, Post-secondary

Collection:

Association for Biology Laboratory Education (ABLE)